Anti-GST3 / GST pi antibody (ab153949)
Key features and details
- Rabbit polyclonal to GST3 / GST pi
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-GST3 / GST pi antibody
See all GST3 / GST pi primary antibodies -
Description
Rabbit polyclonal to GST3 / GST pi -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-176 of Human GST3/ GST pi (Uniprot ID P09211).
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Positive control
- Recombinant Human GST3 / GST pi protein (ab86703) can be used as a positive control in WB. HeLa, Molt-4, mouse brain and rat brain whole cell lysates. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 2950 Human
- Entrez Gene: 14870 Mouse
- Entrez Gene: 24426 Rat
- Omim: 134660 Human
- SwissProt: P09211 Human
- SwissProt: P19157 Mouse
- SwissProt: P04906 Rat
- Unigene: 523836 Human
see all -
Alternative names
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
Images
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All lanes : Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS PAGE -
Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue: Hoechst 33343 staining
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Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12 % SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab153949 has been referenced in 7 publications.
- Kim HN et al. The thrombin receptor modulates astroglia-neuron trophic coupling and neural repair after spinal cord injury. Glia 69:2111-2132 (2021). PubMed: 33887067
- Langley MR et al. Critical Role of Astrocyte NAD+ Glycohydrolase in Myelin Injury and Regeneration. J Neurosci 41:8644-8667 (2021). PubMed: 34493542
- Yang J et al. MicroRNA-212-3p inhibits paclitaxel resistance through regulating epithelial-mesenchymal transition, migration and invasion by targeting ZEB2 in human hepatocellular carcinoma. Oncol Lett 20:23 (2020). PubMed: 32774496
- Chen JL et al. Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation. Ther Adv Med Oncol 11:1758835919838958 (2019). PubMed: 31019568
- Hadami K et al. Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer. Mol Genet Genomic Med 6:819-827 (2018). PubMed: 30043549
- Ma Q et al. Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma. Cancer Biol Ther 18:177-185 (2017). PubMed: 28278080
- McDonagh B et al. Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. J Proteome Res 13:5008-21 (2014). WB ; Mouse . PubMed: 25181601