Anti-GST3 / GST pi antibody (ab153949)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-GST3 / GST pi antibody
See all GST3 / GST pi primary antibodies -
Description
Rabbit polyclonal to GST3 / GST pi -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-176 of Human GST3/ GST pi (Uniprot ID P09211).
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Positive control
- Recombinant Human GST3 / GST pi protein (ab86703) can be used as a positive control in WB. HeLa, Molt-4, mouse brain and rat brain whole cell lysates. HeLa cells.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 2950 Human
- Entrez Gene: 14870 Mouse
- Entrez Gene: 24426 Rat
- Omim: 134660 Human
- SwissProt: P09211 Human
- SwissProt: P19157 Mouse
- SwissProt: P04906 Rat
- Unigene: 523836 Human
see all -
Alternative names
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
Images
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Western blot - Anti-GST3 / GST pi antibody (ab153949)All lanes : Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (ab153949)Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue: Hoechst 33343 staining -
Western blot - Anti-GST3 / GST pi antibody (ab153949)Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Western blot - Anti-GST3 / GST pi antibody (ab153949)Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (ab153949)Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Hadami K et al. Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer. Mol Genet Genomic Med 6:819-827 (2018). Read more (PubMed: 30043549) »
- Ma Q et al. Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma. Cancer Biol Ther 18:177-185 (2017). Read more (PubMed: 28278080) »
