Anti-GST3 / GST pi antibody (ab153949)
Key features and details
- Rabbit polyclonal to GST3 / GST pi
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GST3 / GST pi antibody
See all GST3 / GST pi primary antibodies -
Description
Rabbit polyclonal to GST3 / GST pi -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-176 of Human GST3/ GST pi (Uniprot ID P09211).
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Positive control
- Recombinant Human GST3 / GST pi protein (ab86703) can be used as a positive control in WB. HeLa, Molt-4, mouse brain and rat brain whole cell lysates. HeLa cells.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab153949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 2950 Human
- Entrez Gene: 14870 Mouse
- Entrez Gene: 24426 Rat
- Omim: 134660 Human
- SwissProt: P09211 Human
- SwissProt: P19157 Mouse
- SwissProt: P04906 Rat
- Unigene: 523836 Human
see all -
Alternative names
- Deafness antibody
- Deafness X-linked 7 antibody
- DFN7 antibody
see all
Images
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Western blot - Anti-GST3 / GST pi antibody (ab153949)All lanes : Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-GST3 / GST pi antibody (ab153949)Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue: Hoechst 33343 staining -
Western blot - Anti-GST3 / GST pi antibody (ab153949)Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Western blot - Anti-GST3 / GST pi antibody (ab153949)Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 23 kDa
12 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GST3 / GST pi antibody (ab153949)Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.
Protocols
References (4)
ab153949 has been referenced in 4 publications.
- Chen JL et al. Overexpression of long noncoding RNA LINC01419 in esophageal squamous cell carcinoma and its relation to the sensitivity to 5-fluorouracil by mediating GSTP1 methylation. Ther Adv Med Oncol 11:1758835919838958 (2019). PubMed: 31019568
- Hadami K et al. Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer. Mol Genet Genomic Med 6:819-827 (2018). PubMed: 30043549
- Ma Q et al. Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma. Cancer Biol Ther 18:177-185 (2017). PubMed: 28278080
- McDonagh B et al. Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. J Proteome Res 13:5008-21 (2014). WB ; Mouse . PubMed: 25181601
