Overview

  • Product name

    Anti-GST3 / GST pi antibody
    See all GST3 / GST pi primary antibodies
  • Description

    Rabbit polyclonal to GST3 / GST pi
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-176 of Human GST3/ GST pi (Uniprot ID P09211).

  • Positive control

    • Recombinant Human GST3 / GST pi protein (ab86703) can be used as a positive control in WB. HeLa, Molt-4, mouse brain and rat brain whole cell lysates. HeLa cells.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab153949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 23 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Sequence similarities

    Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Images

  • All lanes : Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate

    Lane 2 : Molt-4 whole cell lysate


    Lysates/proteins at 30 µg per lane.

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Immunofluorescent analysis methanol fixed HeLa cells; labeling GST3 / GST pi with ab153949 at 1/200 dilution. Blue: Hoechst 33343 staining
  • Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 23 kDa



    12% SDS PAGE
  • Anti-GST3 / GST pi antibody (ab153949) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 23 kDa



    12 % SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded U87 xenograft, labeling GST3 / GST pi with ab153949 at 1/500 dilution.

References

This product has been referenced in:

  • Hadami K  et al. Evaluation of glutathione S-transferase pi 1 expression and gene promoter methylation in Moroccan patients with urothelial bladder cancer. Mol Genet Genomic Med 6:819-827 (2018). Read more (PubMed: 30043549) »
  • Ma Q  et al. Hypoxia promotes chemotherapy resistance by down-regulating SKA1 gene expression in human osteosarcoma. Cancer Biol Ther 18:177-185 (2017). Read more (PubMed: 28278080) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 18 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 18 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 8.5
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Brandon Jun

Verified customer

Submitted Dec 29 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 04 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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