Product nameAnti-GSTA1 antibody
See all GSTA1 primary antibodies
DescriptionGoat polyclonal to GSTA1
SpecificityThe antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk).
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Hamster, Human
Full length native protein (purified) corresponding to Rat GSTA1.
Database link: P00502
Previously labelled as Glutathione S Transferase alpha 1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
Purification notesIgG fraction was prepared from the antisera using the caprylic acid precipitation method.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab53940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Sequence similaritiesBelongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
DomainThe C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
- Information by UniProt
- Glutathione S alkyltransferase A1 antibody
- Glutathione S aryltransferase A1 antibody
- Glutathione S transferase 2 antibody
Lane 1. Molecular Weight Marker
Lane 2. GST α Standard
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.
A. Negative control (no antibody).
B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.
ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Liu C et al. MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int 18:54 (2018). WB, IHC-P ; Human . Read more (PubMed: 29632436) »
- Liu H et al. Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16:467-474 (2018). Read more (PubMed: 29928434) »