Anti-GSTA1 antibody (ab53940)

Submit a review Q&A (2)References (6)
Western blot - Anti-GSTA1 antibody (ab53940)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)
  • Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (ab53940)

Key features and details

  • Goat polyclonal to GSTA1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GSTA1 antibody
    See all GSTA1 primary antibodies

  • Description

    Goat polyclonal to GSTA1

  • Host species

    Goat

  • Specificity

    The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk).

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Rat
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Rat GSTA1.
    Database link: P00502

  • General notes

    Previously labelled as Glutathione S Transferase alpha 1.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Rat
Application Abreviews Notes
IHC-P
1/1000.
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.

 
Notes
IHC-P
1/1000.
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).

5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.

 

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

  • Tissue specificity

    Liver.

  • Sequence similarities

    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Domain

    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glutathione S alkyltransferase A1 antibody
      • Glutathione S aryltransferase A1 antibody
      • Glutathione S transferase 2 antibody
      • Glutathione S transferase A1 antibody
      • Glutathione S transferase alpha 1 antibody
      • Glutathione S transferase Ha subunit 1 antibody
      • Glutathione S-transferase A1 antibody
      • GST class-alpha member 1 antibody
      • GST epsilon antibody
      • GST HA subunit 1 antibody
      • GST, class alpha, 1 antibody
      • GST-epsilon antibody
      • GST2 antibody
      • GSTA1 1 antibody
      • GSTA1 antibody
      • GSTA1-1 antibody
      • GSTA1_HUMAN antibody
      • GTH1 antibody
      • HA subunit 1 antibody
      • MGC131939 antibody
      • OTTHUMP00000016611 antibody
      • S (hydroxyalkyl)glutathione lyase A1 antibody
      see all

    Images

    • Western blot - Anti-GSTA1 antibody (ab53940)
      Western blot - Anti-GSTA1 antibody (ab53940)
      Lane 1. Molecular Weight Marker
      Lane 2. GST α Standard

      A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)

      A. Negative control (no antibody).

      B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.

    • Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (ab53940)
      Immunocytochemistry/ Immunofluorescence - Anti-GSTA1 antibody (ab53940)
      ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (6)

    Publishing research using ab53940? Please let us know so that we can cite the reference in this datasheet.

    ab53940 has been referenced in 6 publications.

    • Liu C  et al. MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int 18:54 (2018). WB, IHC-P ; Human . PubMed: 29632436
    • Liu H  et al. Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16:467-474 (2018). PubMed: 29928434
    • Singh A  et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). PubMed: 28289414
    • Grootaert MO  et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy 11:2014-2032 (2015). WB ; Mouse . PubMed: 26391655
    • Volkova M  et al. Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart. Cardiovasc Res 90:305-14 (2011). WB ; Rat . PubMed: 21233252
    • Azkargorta M  et al. Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184-94 (2010). WB ; Mouse . PubMed: 20573986

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Hi, Description of the problem
    multiple bands formed, and the bands look much more distinct compared to the desired band.

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    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
    Reviewing this case, I would like to offer some suggestions to help optimise the results from ab53940. I would also appreciate if you can confirm some further details:
    1) The lysis buffer seems unusual (we usually recommend RIPA buffer), but if it works for you it is fine. However, we strongly recommend adding freshly prepared protease inhibitors. Degradation of proteins can occur very quickly, leading to an unspecific banding pattern which might explain the results you observe. For details see our WB guide attached.
    2) We usually recommend 20-30 ug per lane, so the 30 ug you loaded should be absolutely fine to avoid overloading which might increase unspecific signals as well.
    3) When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. For unknown reasons some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands. An example of the above is the western blot obtained with the Abcam GAPDH antibody ab9385 : https://www.abcam.com/gapdh-antibody-hrp-loading-control-ab9385.html#GAPDH-Primary-antibodies-ab9385-3.jpg (or use the following: www.abcam.com/ab9385). Also, it is then recommended to use the same blocking reagent also in the antibody dilution buffers (i.e. no mixing of milk/BSA).
    4) Have you performed a no primary control to see if the additional bands are caused by unspecific binding of the secondary antibody?
    I hope some of these suggestions will improve the results. If the antibody was purchased in the past 6 months you are covered by our Abpromise guarantee. Therefore if the suggestions do not improve the results I would be happy to offer a refund or replacement vial. Please do not hesitate to contact me should you have additional questions. Thank you for your cooperation.

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    Question

    i purchased the GSTA-1 antibody from your company. recently, i run my western blot and i get a specific band at˜20-26kda. but at the same time i get multiple non specific bands which are more intense compared to the specific bands. i tried with increase with either the protein amount or antibody concentration, but yet it doesnt improve the result.can i noe is there any solution to solve this problem?? or any of your customer encounter this problem before and how they solve it? thank you for your help...thank you and have a nice day!

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    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this Glutathione S Transferase alpha antibody.
    The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. We have received no complaints about this product so far and therefore I am concerned it is not working for you.
    Non specific bands can be caused by various factors, and I would like to investigate this particular case further for you, and also obtain more information for our quality records. In order to proceed with this, I have enclosed a questionnaire. I would appreciate if you could complete this. It will help you put the information we require together very easily.
    I would appreciate if you could also provide an image which would help us to assess the results.
    In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.
    Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

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