Anti-GSTA1 antibody (ab53940)
Key features and details
- Goat polyclonal to GSTA1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-GSTA1 antibody
See all GSTA1 primary antibodies -
Description
Goat polyclonal to GSTA1 -
Host species
Goat -
Specificity
The antibody is known to be class-specific and recognizes alpha-form GSTs from rat, mouse, hamster, rabbit, and human. Recognizes GST A1-1(Ya1), A2-2 (Ya2,Yc2), A3-3 (Ya3,Yc), A4-4 (Yk). -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Rat -
Immunogen
Full length native protein (purified) corresponding to Rat GSTA1.
Database link: P00502 -
General notes
Previously labelled as Glutathione S Transferase alpha 1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
-
Purity
IgG fraction -
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Rat
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/1000.
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
WB |
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.
|
Notes |
---|
IHC-P
1/1000. |
ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). 5 μL per 10 mL of 5% milk powder in TTBS (1:2000 dilution) is recommended for ECL method with overnight incubation at room temperature.
|
Target
-
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Domain
The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 2938 Human
- Entrez Gene: 24421 Rat
- Omim: 138359 Human
- SwissProt: P08263 Human
- SwissProt: P00502 Rat
- Unigene: 446309 Human
-
Alternative names
- Glutathione S alkyltransferase A1 antibody
- Glutathione S aryltransferase A1 antibody
- Glutathione S transferase 2 antibody
see all
Images
-
Lane 1. Molecular Weight Marker
Lane 2. GST α Standard
A 1:2000 dilution is recommended for ECL method with overnight incubation at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTA1 antibody (ab53940)
A. Negative control (no antibody).
B. Rat liver section stained with ab53940 at 1/1000 dilution followed by anti-goat IgG-HRP with DAB HRP substrate.
-
ICC/IF image of ab53940 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53940, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (6)
ab53940 has been referenced in 6 publications.
- Liu C et al. MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int 18:54 (2018). WB, IHC-P ; Human . PubMed: 29632436
- Liu H et al. Downregulation of Glutathione S-transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16:467-474 (2018). PubMed: 29928434
- Singh A et al. Podophyllotoxin and Rutin Modulates Ionizing Radiation-Induced Oxidative Stress and Apoptotic Cell Death in Mice Bone Marrow and Spleen. Front Immunol 8:183 (2017). PubMed: 28289414
- Grootaert MO et al. Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis. Autophagy 11:2014-2032 (2015). WB ; Mouse . PubMed: 26391655
- Volkova M et al. Activation of the aryl hydrocarbon receptor by doxorubicin mediates cytoprotective effects in the heart. Cardiovasc Res 90:305-14 (2011). WB ; Rat . PubMed: 21233252
- Azkargorta M et al. Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes. Mol Cell Proteomics 9:2184-94 (2010). WB ; Mouse . PubMed: 20573986