Overview

  • Product name

    Anti-GSTA1 antibody [EPR19969]
    See all GSTA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19969] to GSTA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GSTA1 aa 1-100. The exact sequence is proprietary.
    Database link: P08263

  • Positive control

    • WB: Human GSTA1 full length recombinant protein; HepG2 and Caco-2 whole cell lysates; Human pancreas, fetal lung, fetal liver, testis and fetal kidney lysates. IP: HepG2 whole cell lysate.
  • General notes

    Previously labelled as Glutathione S Transferase alpha 1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IP 1/30.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • Tissue specificity

    Liver.
  • Sequence similarities

    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Domain

    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glutathione S alkyltransferase A1 antibody
    • Glutathione S aryltransferase A1 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A1 antibody
    • Glutathione S transferase alpha 1 antibody
    • Glutathione S transferase Ha subunit 1 antibody
    • Glutathione S-transferase A1 antibody
    • GST class-alpha member 1 antibody
    • GST epsilon antibody
    • GST HA subunit 1 antibody
    • GST, class alpha, 1 antibody
    • GST-epsilon antibody
    • GST2 antibody
    • GSTA1 1 antibody
    • GSTA1 antibody
    • GSTA1-1 antibody
    • GSTA1_HUMAN antibody
    • GTH1 antibody
    • HA subunit 1 antibody
    • MGC131939 antibody
    • OTTHUMP00000016611 antibody
    • S (hydroxyalkyl)glutathione lyase A1 antibody
    see all

Images

  • All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution

    Lane 1 : Human GSTA1 full length recombinant protein
    Lane 2 : Human GSTA2 full length recombinant protein
    Lane 3 : Human GSTA3 full length recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    GSTA1, A2, and A3 are His-Tagged full-length (aa1-222) recombinant proteins.

    GSTA4 and A5 were not recognized by ab207413 (data not shown).

  • GSTA1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207413 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207413 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab207413 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab207413 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : Human pancreas lysate
    Lane 4 : Human fetal lung lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 and 4: 3 minutes; Lane 5 and 6: 1 second.

  • Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Zou M  et al. Glutathione S-transferase isozyme alpha 1 is predominantly involved in the cisplatin resistance of common types of solid cancer. Oncol Rep 41:989-998 (2019). Read more (PubMed: 30431119) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab207413.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up