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RecombinantRabMAb

Recombinant Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)

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Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
  • Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
  • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
  • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
  • Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19969] to GSTA1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-GSTA1 antibody [EPR19969] - BSA and Azide free
    See all GSTA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19969] to GSTA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251473 is the carrier-free version of ab207413.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab251473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IP
Use at an assay dependent concentration.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

  • Tissue specificity

    Liver.

  • Sequence similarities

    Belongs to the GST superfamily. Alpha family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Domain

    The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Glutathione S alkyltransferase A1 antibody
      • Glutathione S aryltransferase A1 antibody
      • Glutathione S transferase 2 antibody
      • Glutathione S transferase A1 antibody
      • Glutathione S transferase alpha 1 antibody
      • Glutathione S transferase Ha subunit 1 antibody
      • Glutathione S-transferase A1 antibody
      • GST class-alpha member 1 antibody
      • GST epsilon antibody
      • GST HA subunit 1 antibody
      • GST, class alpha, 1 antibody
      • GST-epsilon antibody
      • GST2 antibody
      • GSTA1 1 antibody
      • GSTA1 antibody
      • GSTA1-1 antibody
      • GSTA1_HUMAN antibody
      • GTH1 antibody
      • HA subunit 1 antibody
      • MGC131939 antibody
      • OTTHUMP00000016611 antibody
      • S (hydroxyalkyl)glutathione lyase A1 antibody
      see all

    Images

    • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution

      Lane 1 : Human GSTA1 full length recombinant protein
      Lane 2 : Human GSTA2 full length recombinant protein
      Lane 3 : Human GSTA3 full length recombinant protein

      Lysates/proteins at 0.02 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 5 seconds


      This data was developed using ab207413, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      GSTA1, A2, and A3 are His-Tagged full-length (aa1-222) recombinant proteins.

      GSTA4 and A5 were not recognized by ab207413 (data not shown).

    • Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Immunoprecipitation - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      This data was developed using ab207413, the same antibody clone in a different buffer formulation.GSTA1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207413 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207413 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: HepG2 whole cell lysate, 10µg (Input). Lane 2: ab207413 IP in HepG2 whole cell lysate. Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab207413 in HepG2 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
    • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      All lanes : Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/5000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 3 : Human pancreas lysate
      Lane 4 : Human fetal lung lysate
      Lane 5 : Human fetal liver lysate
      Lane 6 : Human testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 3-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa



      This data was developed using ab207413, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: Lanes 1-4: 3 minutes; Lanes 5 and 6: 1 second.

    • Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Western blot - Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Anti-GSTA1 antibody [EPR19969] (ab207413) at 1/2000 dilution + Human fetal kidney lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa


      Exposure time: 3 minutes


      This data was developed using ab207413, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)
      Anti-GSTA1 antibody [EPR19969] - BSA and Azide free (ab251473)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251473? Please let us know so that we can cite the reference in this datasheet.

    ab251473 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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