Key features and details
- Rabbit polyclonal to GSTA4
- Suitable for: IHC-P, WB
- Reacts with: Rat
Product nameAnti-GSTA4 antibody
See all GSTA4 primary antibodies
DescriptionRabbit polyclonal to GSTA4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse
Recombinant full length protein (His-T7-tag) corresponding to Rat GSTA4 aa 2-222. Expressed in E.coli. N-terminal tags.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEVKPKLYYFQGRGRME SIRWLLATAGVEFEEEFLETREQYEKLQKDGCLLFGQVPLVEIDGMLLTQ TRAILSYLAAKYNLYGKDLKERVRIDMYADGTQDLMMMIIGAPFKAPQEK EESLALAVKRAKNRYFPVFEKILKDHGEAFLVGNQLSWADIQLLEAILMV EEVSAPVLSDFPLLQAFKTRISNIPTIKKFLQPGSQRKPPPDGHYVDVVR TVLKF
Database link: P14942
- WB: Recombinant rat GSTA4 protein; rat serum; rat brain and liver lysates. IHC-P: Rat colon, testis, kidney, lung and liver tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab231601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.|
FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
Tissue specificityExpressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver.
Sequence similaritiesBelongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
- Information by UniProt
- DKFZp686D21185 antibody
- Glutathione S alkyltransferase A4 antibody
- Glutathione S aralkyltransferase A4 antibody
All lanes : Anti-GSTA4 antibody (ab231601) at 3 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat brain lysate
Lane 3 : Rat liver lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Formalin-fixed, paraffin-embedded rat colon tissue stained for GSTA4 using ab231601 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat testis tissue stained for GSTA4 using ab231601 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat kidney tissue stained for GSTA4 using ab231601 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat liver tissue stained for GSTA4 using ab231601 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded rat lung tissue stained for GSTA4 using ab231601 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-GSTA4 antibody (ab231601) at 3 µg/ml + Recombinant rat GSTA4 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231601 has not yet been referenced specifically in any publications.