Recombinant
RabMAb

Recombinant Anti-GSTA4 antibody [EPR8404(B)] (ab134919)

Overview

  • Product name

    Anti-GSTA4 antibody [EPR8404(B)]
    See all GSTA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8404(B)] to GSTA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human GSTA4 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, fetal liver, 293T, HepG2 and JAR cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
    • Tissue specificity

      Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver.
    • Sequence similarities

      Belongs to the GST superfamily. Alpha family.
      Contains 1 GST C-terminal domain.
      Contains 1 GST N-terminal domain.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp686D21185 antibody
      • Glutathione S alkyltransferase A4 antibody
      • Glutathione S aralkyltransferase A4 antibody
      • Glutathione S aryltransferase A4 antibody
      • Glutathione S transferase A4 4 antibody
      • Glutathione S transferase A4 antibody
      • Glutathione S transferase alpha 4 antibody
      • Glutathione S-transferase A4 antibody
      • Glutathione S-transferase A4-4 antibody
      • Glutathione transferase A4 4 antibody
      • GST class alpha member 4 antibody
      • GST class-alpha member 4 antibody
      • GSTA4 4 antibody
      • GSTA4 antibody
      • GSTA4_HUMAN antibody
      • GTA4 antibody
      • OTTHUMP00000016624 antibody
      • OTTHUMP00000016625 antibody
      • S (hydroxyalkyl)glutathione lyase A4 antibody
      see all

    Images

    • All lanes : Anti-GSTA4 antibody [EPR8404(B)] (ab134919) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 26 kDa

    References

    ab134919 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293Tcells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    20 µg
    Specification
    293Tcells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up