Recombinant Anti-GSTA4 antibody [EPR8404(B)] (ab134919)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8404(B)] to GSTA4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GSTA4 antibody [EPR8404(B)]
See all GSTA4 primary antibodies -
Description
Rabbit monoclonal [EPR8404(B)] to GSTA4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human GSTA4 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- Human fetal brain, fetal liver, 293T, HepG2 and JAR cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8404(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE). -
Tissue specificity
Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. -
Sequence similarities
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2941 Human
- Entrez Gene: 300850 Rat
- Omim: 605450 Human
- SwissProt: O15217 Human
- SwissProt: P14942 Rat
- Unigene: 485557 Human
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Alternative names
- DKFZp686D21185 antibody
- Glutathione S alkyltransferase A4 antibody
- Glutathione S aralkyltransferase A4 antibody
see all
Images
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All lanes : Anti-GSTA4 antibody [EPR8404(B)] (ab134919) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal liver lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134919 has been referenced in 1 publication.
- Kim JK et al. UHRF1 downmodulation enhances antitumor effects of histone deacetylase inhibitors in retinoblastoma by augmenting oxidative stress-mediated apoptosis. Mol Oncol 14:329-346 (2020). PubMed: 31782885