Overview

  • Product name

  • Description

    Rabbit polyclonal to GSTK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GSTK1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9Y2Q3

  • Positive control

    • WB: HepG2 whole cell lysate. IHC-P: Human lung carcinoma and gastric cancer tissues. ICC/IF: A549 cells.
  • General notes

    Previously labelled as Glutathione S Transferase kappa 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab229219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000.

Target

  • Function

    Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the GST superfamily. Kappa family.
  • Cellular localization

    Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.5.1.18 antibody
    • Glutathione S Transferase kappa 1 antibody
    • Glutathione S transferase subunit 13 antibody
    • Glutathione S-transferase k1 antibody
    • Glutathione S-transferase kappa 1 antibody
    • Glutathione S-transferase subunit 13 antibody
    • Glutathione S-transferase subunit 13 homolog antibody
    • GST 13 13 antibody
    • GST 13-13 antibody
    • GST antibody
    • GST class kappa antibody
    • GST class-kappa antibody
    • GST13 antibody
    • GST13-13 antibody
    • GSTK1 1 antibody
    • Gstk1 antibody
    • GSTK1-1 antibody
    • GSTK1_HUMAN antibody
    • hGSTK1 antibody
    see all

Images

  • All lanes : Anti-GSTK1 antibody (ab229219) at 1/1000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : GSTK1 Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG
  • Paraffin-embedded human lung carcinoma tissue stained for GSTK1 using ab229219 at 1/100 dilution in immunohistochemical analysis.

  • Anti-GSTK1 antibody (ab229219) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg


    12% SDS-PAGE gel.

  • Paraffin-embedded human gastric cancer tissue stained for GSTK1 using ab229219 at 1/500 dilution in immunohistochemical analysis.

  • Methanol-fixed A549 (human lung carcinoma cell line) cells stained for GSTK1 (green) using ab229219 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229219 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229219.
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