Product nameAnti-GSTK1 antibody
See all GSTK1 primary antibodies
DescriptionRabbit polyclonal to GSTK1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human GSTK1 (internal sequence). The exact sequence is proprietary.
Database link: Q9Y2Q3
- WB: HepG2 whole cell lysate. IHC-P: Human lung carcinoma and gastric cancer tissues. ICC/IF: A549 cells.
Previously labelled as Glutathione S Transferase kappa 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab229219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000.|
FunctionSignificant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).
Sequence similaritiesBelongs to the GST superfamily. Kappa family.
- Information by UniProt
- EC 220.127.116.11 antibody
- Glutathione S Transferase kappa 1 antibody
- Glutathione S transferase subunit 13 antibody
All lanes : Anti-GSTK1 antibody (ab229219) at 1/1000 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : GSTK1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Paraffin-embedded human lung carcinoma tissue stained for GSTK1 using ab229219 at 1/100 dilution in immunohistochemical analysis.
Anti-GSTK1 antibody (ab229219) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
12% SDS-PAGE gel.
Paraffin-embedded human gastric cancer tissue stained for GSTK1 using ab229219 at 1/500 dilution in immunohistochemical analysis.
Methanol-fixed A549 (human lung carcinoma cell line) cells stained for GSTK1 (green) using ab229219 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab229219 has not yet been referenced specifically in any publications.