Anti-GSTK1 antibody (ab231549)
Key features and details
- Rabbit polyclonal to GSTK1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GSTK1 antibody
See all GSTK1 primary antibodies -
Description
Rabbit polyclonal to GSTK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse GSTK1 aa 1 to the C-terminus. N-terminal tags. Expressed in E.coli.
Database link: Q9DCM2 -
Positive control
- IHC-P: Mouse liver, kidney, brain and stomach tissues. WB: HeLa and Raji cell lysates; Mouse liver and heart lysates; Recombinant human GSTK1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231549 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the GST superfamily. Kappa family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 373156 Human
- Entrez Gene: 76263 Mouse
- Omim: 602321 Human
- SwissProt: Q9Y2Q3 Human
- SwissProt: Q9DCM2 Mouse
- Unigene: 390667 Human
- Unigene: 267014 Mouse
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Alternative names
- EC 2.5.1.18 antibody
- Glutathione S Transferase kappa 1 antibody
- Glutathione S transferase subunit 13 antibody
see all
Images
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Paraffin-embedded mouse liver tissue stained for GSTK1 using ab231549 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse kidney tissue stained for GSTK1 using ab231549 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse brain tissue stained for GSTK1 using ab231549 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded mouse stomach tissue stained for GSTK1 using ab231549 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-GSTK1 antibody (ab231549) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : Mouse liver lysate
Lane 4 : Mouse heart lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
Anti-GSTK1 antibody (ab231549) at 3 µg/ml + Recombinant human GSTK1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231549 has not yet been referenced specifically in any publications.