Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GSTK1 antibody [EPR1939] (ab134173)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1939] to GSTK1
  • Suitable for: IHC-P, WB, IP, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GSTK1 antibody [EPR1939]
    See all GSTK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1939] to GSTK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IP, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human GSTK1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Daudi, HeLa and HEK-293 cell lysates IHC-P Human kidney tissue ICC/IF: HEK-293 cells Flow Cyt: HeLa Cells IP: HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Glutathione S Transferase kappa 1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab134173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/300. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
WB 1/1000. Predicted molecular weight: 25 kDa.
IP 1/30.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50.

Target

  • Function

    Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the GST superfamily. Kappa family.

  • Cellular localization

    Peroxisome.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.5.1.18 antibody
      • Glutathione S Transferase kappa 1 antibody
      • Glutathione S transferase subunit 13 antibody
      • Glutathione S-transferase k1 antibody
      • Glutathione S-transferase kappa 1 antibody
      • Glutathione S-transferase subunit 13 antibody
      • Glutathione S-transferase subunit 13 homolog antibody
      • GST 13 13 antibody
      • GST 13-13 antibody
      • GST antibody
      • GST class kappa antibody
      • GST class-kappa antibody
      • GST13 antibody
      • GST13-13 antibody
      • GSTK1 1 antibody
      • Gstk1 antibody
      • GSTK1-1 antibody
      • GSTK1_HUMAN antibody
      • hGSTK1 antibody
      see all

    Images

    • Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)
      All lanes : Anti-GSTK1 antibody [EPR1939] (ab134173) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : GSTK1 knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 25 kDa
      Observed band size: 27 kDa
      why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab134173 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab134173 Anti-GSTK1 antibody [EPR1939] was shown to specifically react with GSTK1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266112 (knockout cell lysate ab257461) was used.  Wild-type and GSTK1 knockout samples were subjected to SDS-PAGE.  ab134173 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)
      All lanes : Anti-GSTK1 antibody [EPR1939] (ab134173) at 1/1000 dilution (Purified)

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking/Diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EPR1939] (ab134173)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling GSTK1 with purified ab134173 at 1/300 dilution (0.99 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling GSTK1 with Purified ab134173 at 1/50 dilution (5.92 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunoprecipitation - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Immunoprecipitation - Anti-GSTK1 antibody [EPR1939] (ab134173)

      Purified ab134173 at 1/30 dilution (2ug) immunoprecipitating GSTK1 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (10µg)
      Lane 2 (+): ab134173 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134173 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 25 kDa

    • Flow Cytometry - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Flow Cytometry - Anti-GSTK1 antibody [EPR1939] (ab134173)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GSTK1 with Purified ab134173 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    References

    Publishing research using ab134173? Please let us know so that we can cite the reference in this datasheet.

    ab134173 has not yet been referenced specifically in any publications.

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