Recombinant Anti-GSTK1 antibody [EPR1939] (ab134173)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GSTK1 antibody [EPR1939]
See all GSTK1 primary antibodies -
Description
Rabbit monoclonal [EPR1939] to GSTK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GSTK1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Human liver tissue; HeLa cells; Human fetal liver, Human heart, 293T and HeLa lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Glutathione S Transferase kappa 1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1939 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 25 kDa. | |
| IP | 1/30. | |
| Flow Cyt | 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/250 - 1/500. |
Target
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Function
Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the GST superfamily. Kappa family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 373156 Human
- Omim: 602321 Human
- SwissProt: Q9Y2Q3 Human
- Unigene: 390667 Human
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Alternative names
- EC 2.5.1.18 antibody
- Glutathione S Transferase kappa 1 antibody
- Glutathione S transferase subunit 13 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EPR1939] (ab134173)](https://www.abcam.com/ps/products/134/ab134173/Images/ab134173-148483-anti-gstk1-antibody-epr1939-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTK1 antibody [EPR1939] (ab134173)Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling GSTK1 with ab134173 at 1/250 dilution.
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![Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)](https://www.abcam.com/ps/products/134/ab134173/Images/ab134173-148485-anti-gstk1-antibody-epr1939-western-blot.jpg)
Western blot - Anti-GSTK1 antibody [EPR1939] (ab134173)All lanes : Anti-GSTK1 antibody [EPR1939] (ab134173) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human heart lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EPR1939] (ab134173)](https://www.abcam.com/ps/products/134/ab134173/Images/ab134173-148484-anti-gstk1-antibody-epr1939-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GSTK1 antibody [EPR1939] (ab134173)Immunofluorescent analysis of HeLa cells labelling GSTK1 with ab134173 at 1/250 dilution.
Protocols
Datasheets and documents
References
ab134173 has not yet been referenced specifically in any publications.
