Overview

  • Product name

  • Description

    Goat polyclonal to GSTM4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human GSTM4 aa 106-119 (internal sequence). (NP_000841.1).
    Sequence:

    DVSNQLARVCYSPD


    Database link: Q03013

  • Positive control

    • WB: HeLa whole cell lysate (ab150035); Rat lung lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223480 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene.
  • Tissue specificity

    Expressed in a wide variety of tissues.
  • Sequence similarities

    Belongs to the GST superfamily. Mu family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Glutathione S transferase M4 antibody
    • Glutathione S transferase Mu 4 antibody
    • Glutathione S-transferase Mu 4 antibody
    • GST class mu 4 antibody
    • GST class-mu 4 antibody
    • GST-Mu2 antibody
    • GSTM4 4 antibody
    • GSTM4 antibody
    • GSTM4-4 antibody
    • GSTM4_HUMAN antibody
    • GTM4 antibody
    • GTS Mu2 antibody
    • MGC131945 antibody
    • MGC9247 antibody
    see all

Images

  • Anti-GSTM4 antibody (ab223480) at 0.1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Primary incubation was 1 hour.

  • ab223480 (5 µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Anti-GSTM4 antibody (ab223480) at 0.3 µg/ml + Rat lung lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Primary incubation was 1 hour.

References

ab223480 has not yet been referenced specifically in any publications.

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