Anti-GSTT1 antibody (ab175418)

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Overview

  • Product name

    Anti-GSTT1 antibody
    See all GSTT1 primary antibodies

  • Description

    Rabbit polyclonal to GSTT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Recombinant full length protein corresponding to Human GSTT1 aa 1-240.
    Sequence:

    MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKGQHLSDAFAQVNP LKKVPALKDGDFTLTESVAILLYLTRKYKVPDYWYPQDLQARARVDEYLA WQHTTLRRSCLRALWHKVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDK FLQNKAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEA AVGEDLFQEAHEVILKAKDFPPADPTIKQKLMPWVLAMIR


    Database link: P30711

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HepG2 cell extract.

  • General notes

     This product was previously labelled as Glutathione S Transferase theta 1

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 27 kDa.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

  • Tissue specificity

    Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

  • Sequence similarities

    Belongs to the GST superfamily. Theta family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 2.5.1.18 antibody
    • Glutathione S transferase 5 antibody
    • Glutathione S transferase theta 1 antibody
    • Glutathione S-transferase theta-1 antibody
    • Glutathione transferase T1 1 antibody
    • Glutathione transferase T1-1 antibody
    • GST 5 5 antibody
    • GST CL1 antibody
    • GST class theta 1 antibody
    • GST class-theta-1 antibody
    • GSTT1 antibody
    • GSTT1_HUMAN antibody
    see all

Images

  • Western blot - Anti-GSTT1 antibody (ab175418)
    Western blot - Anti-GSTT1 antibody (ab175418)
    Anti-GSTT1 antibody (ab175418) at 1/500 dilution + HepG2 cell extract

    Predicted band size: 27 kDa

References

This product has been referenced in:

  • Xie MY  et al. 5-aza-2'-deoxycytidine in the regulation of antioxidant enzymes in retinal endothelial cells and rat diabetic retina. Int J Ophthalmol 12:1-7 (2019). Read more (PubMed: 30662833) »
See 1 Publication for this product

Publishing research using ab175418? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hello! I am looking to buy a human GSTT1 primary antibody for western blotting. I wish to avoid cross-reactivity with GSTT2 and am looking for an antibody that preferably binds to the C-terminus.

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Answer

The Anti-Glutathione S Transferase theta 1 antibody (ab81033) has not been tested for cross-reactivity with the GSTT2 protein. The short immunogen peptide (˜14 aa) used to raise this antibody has 100% homology to human GSTT1 protein. The immunogen peptide has no homology/ similarity to the human GSTT2 protein, therefore it is safe to say that it will not be cross-reactive.

Unfortunately, the Anti-Glutathione S Transferase theta 1 antibody (ab96592) has not been tested in any cross-reactivity experiment to determine the specificity of the antibody for GSTT1. The homology identity of the ab96592 immunogen to GSTT2 is 54%. Therefore there is a chance that the antibody would react with GSTT2.

Unfortunately, the Anti-Glutathione S Transferase theta 1 (ab175418) antibody has not been tested in any cross-reactivity experiment to determine the specificity of the antibody for GSTT1. The molecular weight of these two protein are closed therefore the two proteins cannot be distinguished through WB experiment.

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