RecombinantRabMAb

Recombinant Anti-GSTT1 antibody [EPR19283] (ab199337)

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Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
  • Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)
  • Anti-GSTT1 antibody [EPR19283] (ab199337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19283] to GSTT1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GSTT1 antibody [EPR19283]
    See all GSTT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19283] to GSTT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GSTT1 aa 50-150. The exact sequence is proprietary.
    Database link: P30711

  • Positive control

    • WB: Human fetal kidney and fetal liver lysates; HepG2 whole cell lysate; Mouse lung and liver lysates; Rat lung and liver lysates. IP: HepG2 whole cell lysate.

  • General notes

     

     

     This product was previously labelled as Glutathione S Transferase theta 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19283

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
IP 1/40.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

  • Tissue specificity

    Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

  • Sequence similarities

    Belongs to the GST superfamily. Theta family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 2.5.1.18 antibody
    • Glutathione S transferase 5 antibody
    • Glutathione S transferase theta 1 antibody
    • Glutathione S-transferase theta-1 antibody
    • Glutathione transferase T1 1 antibody
    • Glutathione transferase T1-1 antibody
    • GST 5 5 antibody
    • GST CL1 antibody
    • GST class theta 1 antibody
    • GST class-theta-1 antibody
    • GSTT1 antibody
    • GSTT1_HUMAN antibody
    see all

Images

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID: 24586676).

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Rat lung lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 5 seconds; Lane 4: 30 seconds.

  • Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)

    GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HepG2 whole cell lysate, 10µg (Input).
    Lane 2: ab199337 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Anti-GSTT1 antibody [EPR19283] (ab199337)
    Anti-GSTT1 antibody [EPR19283] (ab199337)

References (0)

Publishing research using ab199337? Please let us know so that we can cite the reference in this datasheet.

ab199337 has not yet been referenced specifically in any publications.

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