Recombinant
RabMAb

Recombinant Anti-GSTT1 antibody [EPR19283] (ab199337)

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Overview

  • Product name

    Anti-GSTT1 antibody [EPR19283]
    See all GSTT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19283] to GSTT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GSTT1 aa 50-150. The exact sequence is proprietary.
    Database link: P30711

  • Positive control

    • WB: Human fetal kidney and fetal liver lysates; HepG2 whole cell lysate; Mouse lung and liver lysates; Rat lung and liver lysates. IP: HepG2 whole cell lysate.

  • General notes

     

     

     This product was previously labelled as Glutathione S Transferase theta 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19283

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
IP 1/40.

Target

  • Function

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.

  • Tissue specificity

    Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

  • Sequence similarities

    Belongs to the GST superfamily. Theta family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EC 2.5.1.18 antibody
    • Glutathione S transferase 5 antibody
    • Glutathione S transferase theta 1 antibody
    • Glutathione S-transferase theta-1 antibody
    • Glutathione transferase T1 1 antibody
    • Glutathione transferase T1-1 antibody
    • GST 5 5 antibody
    • GST CL1 antibody
    • GST class theta 1 antibody
    • GST class-theta-1 antibody
    • GSTT1 antibody
    • GSTT1_HUMAN antibody
    see all

Images

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID: 24586676).

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)
    All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Rat lung lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 5 seconds; Lane 4: 30 seconds.

  • Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)
    Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)

    GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HepG2 whole cell lysate, 10µg (Input).
    Lane 2: ab199337 IP in HepG2 whole cell lysate.
    Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

References

ab199337 has not yet been referenced specifically in any publications.

Publishing research using ab199337? Please let us know so that we can cite the reference in this datasheet.

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