Recombinant Anti-GSTT1 antibody [EPR19283] (ab199337)
![Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)](https://www.abcam.com/ps/products/199/ab199337/Images/ab199337-254694-anti-glutathione-s-transferase-theta-1-antibody-epr19283-western-blot.jpg "Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19283] to GSTT1
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GSTT1 antibody [EPR19283]
See all GSTT1 primary antibodies -
Description
Rabbit monoclonal [EPR19283] to GSTT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal kidney and fetal liver lysates; HepG2 whole cell lysate; Mouse lung and liver lysates; Rat lung and liver lysates. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19283 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).
|
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| IP |
1/40.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa). |
|
IP
1/40. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. -
Tissue specificity
Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles. -
Sequence similarities
Belongs to the GST superfamily. Theta family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2952 Human
- Entrez Gene: 14871 Mouse
- Entrez Gene: 25260 Rat
- Omim: 600436 Human
- SwissProt: P30711 Human
- SwissProt: Q64471 Mouse
- SwissProt: Q01579 Rat
- Unigene: 268573 Human
see all -
Alternative names
- EC 2.5.1.18 antibody
- Glutathione S transferase 5 antibody
- Glutathione S transferase theta 1 antibody
see all
Images
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Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Jurkat doesn't express GSTT1. The expression profile observed is consistent with what has been described in the literature (PMID: 24586676).
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![Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)](https://www.abcam.com/ps/products/199/ab199337/Images/ab199337-254693-anti-glutathione-s-transferase-theta-1-antibody-epr19283-western-blot.jpg)
Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)](https://www.abcam.com/ps/products/199/ab199337/Images/ab199337-254692-anti-glutathione-s-transferase-theta-1-antibody-epr19283-western-blot.jpg)
Western blot - Anti-GSTT1 antibody [EPR19283] (ab199337)All lanes : Anti-GSTT1 antibody [EPR19283] (ab199337) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 3 minutes; Lane 3: 5 seconds; Lane 4: 30 seconds.
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![Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)](https://www.abcam.com/ps/products/199/ab199337/Images/ab199337-254691-anti-glutathione-s-transferase-theta-1-antibody-epr19283-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GSTT1 antibody [EPR19283] (ab199337)GSTT1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab199337 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab199337 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10µg (Input).
Lane 2: ab199337 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] -Isotype Control (ab172730) instead of ab199337 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Anti-GSTT1 antibody [EPR19283] (ab199337)](https://www.abcam.com/ps/products/199/ab199337/Images/ab199337-6-benefits-of-recombinant-antibodies.png)
Anti-GSTT1 antibody [EPR19283] (ab199337)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab199337 has been referenced in 2 publications.
- Poisson J et al. Erythrocyte-derived microvesicles induce arterial spasms in JAK2V617F myeloproliferative neoplasm. J Clin Invest 130:2630-2643 (2020). PubMed: 32045382
- Li D et al. Caloric restriction attenuates C57BL/6?J mouse lung injury and extra-pulmonary toxicity induced by real ambient particulate matter exposure. Part Fibre Toxicol 17:22 (2020). PubMed: 32503629
