Recombinant Anti-GSTT2 antibody [EPR8136(2)] - BSA and Azide free (ab249912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8136(2)] to GSTT2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-GSTT2 antibody [EPR8136(2)] - BSA and Azide free
See all GSTT2 primary antibodies -
Description
Rabbit monoclonal [EPR8136(2)] to GSTT2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human lung tissue lysate and HeLa cell lysate.
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General notes
ab249912 is the carrier-free version of ab176336.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8136(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
Target
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a sulfatase activity. -
Tissue specificity
Expressed at low levels in liver. In lung, expressed at low levels in ciliated bronchiolar cells, alveolar macrophages and alveolar type II cells. -
Sequence similarities
Belongs to the GST superfamily. Theta family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2953 Human
- Entrez Gene: 653689 Human
- Entrez Gene: 14872 Mouse
- Omim: 600437 Human
- SwissProt: P0CG29 Human
- SwissProt: P0CG30 Human
- SwissProt: Q61133 Mouse
- Unigene: 654462 Human
see all -
Alternative names
- AI266894 antibody
- EC 2.5.1.18 antibody
- Glutathione S transferase 12 antibody
see all
Images
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All lanes : Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GSTT2B knockout HeLa cell lysate
Lane 3 : Human lung tissue lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab176336).
Lanes 1-4: Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249912 has not yet been referenced specifically in any publications.