Anti-GSTZ1 antibody (ab153995)

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Overview

  • Product name

    Anti-GSTZ1 antibody
    See all GSTZ1 primary antibodies

  • Description

    Rabbit polyclonal to GSTZ1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Recombinant fragment corresponding to Human GSTZ1 aa 24-216.
    Database link: O43708

  • Positive control

    • 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates. SW480 xenograft; A431 cells.

  • General notes

     This product was previously labelled as Glutathione Transferase zeta 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab153995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.

  • Tissue specificity

    Mostly expressed in liver followed by kidney, skeletal muscle and brain. Also expressed in melanocytes, synovium, placenta, breast and fetal liver and heart.

  • Pathway

    Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.

  • Sequence similarities

    Belongs to the GST superfamily. Zeta family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • EC 2.5.1.18 antibody
      • EC 5.2.1.2 antibody
      • Glutathione S transferase zeta 1 antibody
      • Glutathione S-transferase zeta 1 antibody
      • Gstz1 antibody
      • GSTZ1-1 antibody
      • MAAI antibody
      • MAAI_HUMAN antibody
      • Maleylacetoacetate isomerase antibody
      see all

    Images

    • Western blot - Anti-GSTZ1 antibody (ab153995)
      Western blot - Anti-GSTZ1 antibody (ab153995)
      Anti-GSTZ1 antibody (ab153995) at 1/1000 dilution + 293T cell lysate at 30 µg

      Predicted band size: 24 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)

      Immunohistochemical analysis of paraffin- embedded SW480 xenograft labeling GSTZ1 using ab153995 at 1/100 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)
      Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)

      Immunofluorescence analysis of methanol-fixed A431 cells, labeling GSTZ1 using ab153995 at 1/200 dilution. Right panel costained with Hoechst 33342.

    References

    ab153995 has not yet been referenced specifically in any publications.

    Publishing research using ab153995? Please let us know so that we can cite the reference in this datasheet.

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