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Signal Transduction Metabolism Amino Acids
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Anti-GSTZ1 antibody (ab153995)

  • Datasheet
  • SDS
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Western blot - Anti-GSTZ1 antibody (ab153995)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)
  • Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)

Key features and details

  • Rabbit polyclonal to GSTZ1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human GSTZ1 protein (ab172170)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GSTZ1 antibody
  • Description

    Rabbit polyclonal to GSTZ1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human GSTZ1 aa 24-216.
    Database link: O43708

  • Positive control

    • 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates. SW480 xenograft; A431 cells.
  • General notes

     This product was previously labelled as Glutathione Transferase zeta 1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Tags & Cell Markers
    • Fusion / Marker Proteins
    • GST
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human GSTZ1 protein (ab172170)
  • Related Products

    • Recombinant Human GSTZ1 protein (ab93691)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid.
  • Tissue specificity

    Mostly expressed in liver followed by kidney, skeletal muscle and brain. Also expressed in melanocytes, synovium, placenta, breast and fetal liver and heart.
  • Pathway

    Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6.
  • Sequence similarities

    Belongs to the GST superfamily. Zeta family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O43708 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2954 Human
    • Entrez Gene: 100626791 Pig
    • Omim: 603758 Human
    • SwissProt: O43708 Human
    • Unigene: 655292 Human
    • Alternative names

      • EC 2.5.1.18 antibody
      • EC 5.2.1.2 antibody
      • Glutathione S transferase zeta 1 antibody
      • Glutathione S-transferase zeta 1 antibody
      • Gstz1 antibody
      • GSTZ1-1 antibody
      • MAAI antibody
      • MAAI_HUMAN antibody
      • Maleylacetoacetate isomerase antibody
      see all

    Images

    • Western blot - Anti-GSTZ1 antibody (ab153995)
      Western blot - Anti-GSTZ1 antibody (ab153995)
      Anti-GSTZ1 antibody (ab153995) at 1/1000 dilution + 293T cell lysate at 30 µg

      Predicted band size: 24 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)

      Immunohistochemical analysis of paraffin- embedded SW480 xenograft labeling GSTZ1 using ab153995 at 1/100 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)
      Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)

      Immunofluorescence analysis of methanol-fixed A431 cells, labeling GSTZ1 using ab153995 at 1/200 dilution. Right panel costained with Hoechst 33342.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab153995? Please let us know so that we can cite the reference in this datasheet.

    ab153995 has not yet been referenced specifically in any publications.

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