Anti-GSTZ1 antibody (ab153995)
Key features and details
- Rabbit polyclonal to GSTZ1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GSTZ1 antibody -
Description
Rabbit polyclonal to GSTZ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human GSTZ1 aa 24-216.
Database link: O43708 -
Positive control
- 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates. SW480 xenograft; A431 cells.
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General notes
This product was previously labelled as Glutathione Transferase zeta 1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer
|
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate Buffer |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. -
Tissue specificity
Mostly expressed in liver followed by kidney, skeletal muscle and brain. Also expressed in melanocytes, synovium, placenta, breast and fetal liver and heart. -
Pathway
Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 5/6. -
Sequence similarities
Belongs to the GST superfamily. Zeta family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 2954 Human
- Entrez Gene: 100626791 Pig
- Omim: 603758 Human
- SwissProt: O43708 Human
- Unigene: 655292 Human
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Alternative names
- EC 2.5.1.18 antibody
- EC 5.2.1.2 antibody
- Glutathione S transferase zeta 1 antibody
see all
Images
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Western blot - Anti-GSTZ1 antibody (ab153995)Anti-GSTZ1 antibody (ab153995) at 1/1000 dilution + 293T cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSTZ1 antibody (ab153995)Immunohistochemical analysis of paraffin- embedded SW480 xenograft labeling GSTZ1 using ab153995 at 1/100 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-GSTZ1 antibody (ab153995)Immunofluorescence analysis of methanol-fixed A431 cells, labeling GSTZ1 using ab153995 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153995 has not yet been referenced specifically in any publications.