Overview

  • Product name

    Anti-GTF2E2 antibody [EPR14929]
    See all GTF2E2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14929] to GTF2E2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human GTF2E2 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P29084

  • Positive control

    • K562, Jurkat, 293T, mouse heart, rat heart and rat brain lysates; Human cervix carcinoma and mouse liver tissues; 293T cells.
  • General notes

     

     

     This product was previously labelled as TFIIE beta

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/250.
IP 1/30 - 1/50.

Target

  • Function

    Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • Sequence similarities

    Belongs to the TFIIE beta subunit family.
    Contains 1 TFIIE beta DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FE antibody
    • General transcription factor IIE subunit 2 antibody
    • General transcription factor IIE, polypeptide 2, beta 34kDa antibody
    • gtf2e2 antibody
    • T2EB_HUMAN antibody
    • TF2E 2 antibody
    • TF2E2 antibody
    • TFIIE B antibody
    • TFIIE-beta antibody
    • Transcription initiation factor IIE beta subunit antibody
    • Transcription initiation factor IIE subunit beta antibody
    see all

Images

  • All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/10000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Jurkat lysate
    Lane 3 : 293T lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-GTF2E2 antibody [EPR14929] (ab187143) at 1/1000 dilution

    Lanes 1 & 3 : Mouse heart lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation. ab187143 at 1/50 labeling GTF2E2 immunoprecipitated from Jurkat cell lysate using ab187143 at 1/30.

  • Immunofluorescent analysis of 293T cells labeling GTF2E2 with ab187143 at 1/250 and DAPI staining (blue).

  • Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling GTF2E2 with ab187143 at 1/250 with a prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

  • Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue labeling GTF2E2 with ab187143 at 1/250 with prediluted HRP Polymer for Rabbit IgG as a secondary antibody.

References

This product has been referenced in:

  • Gonçalves E  et al. Widespread Post-transcriptional Attenuation of Genomic Copy-Number Variation in Cancer. Cell Syst 5:386-398.e4 (2017). Read more (PubMed: 29032074) »
  • Theil AF  et al. Trichothiodystrophy causative TFIIEß mutation affects transcription in highly differentiated tissue. Hum Mol Genet 26:4689-4698 (2017). Read more (PubMed: 28973399) »
See all 2 Publications for this product

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