Overview

  • Product name

    Anti-GTF2F2 antibody [EPR7584]
    See all GTF2F2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7584] to GTF2F2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human GTF2F2 (Uniprot: P13984)

  • Positive control

    • HeLa and 293T cell lysates; Human cervical carcinoma tissue; Human colon tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/25 - 1/50.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
    • Sequence similarities

      Belongs to the TFIIF beta subunit family.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATP dependent helicase GTF2F2 antibody
      • ATP-dependent helicase GTF2F2 antibody
      • BTF4 antibody
      • General transcription factor IIF 30 kDa subunit antibody
      • General transcription factor IIF polypeptide 2 antibody
      • General transcription factor IIF subunit 2 antibody
      • General transcription factor IIF, polypeptide 2, 30kDa antibody
      • Gtf2f2 antibody
      • RAP30 antibody
      • T2FB_HUMAN antibody
      • TF2F2 antibody
      • TFIIF antibody
      • TFIIF beta antibody
      • TFIIF-beta antibody
      • Transcription initiation factor IIF subunit beta antibody
      • Transcription initiation factor RAP30 antibody
      see all

    Images

    • All lanes : Anti-GTF2F2 antibody [EPR7584] (ab128945) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?

    • ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human cervical carcinoma tissue by immunohistochemistry.
    • ab128945 at a 1/50 dilution staining GTF2F2 in paraffin-embedded Human colon tissue by immunohistochemistry.

    References

    ab128945 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128945.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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