Anti-GTF2H1 antibody (ab232982)
Key features and details
- Rabbit polyclonal to GTF2H1
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-GTF2H1 antibody
See all GTF2H1 primary antibodies -
Description
Rabbit polyclonal to GTF2H1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human GTF2H1 aa 50-350. N-terminal tags. Expressed in E. coli.
Database link: P32780 -
Positive control
- IHC-P: Human liver tissue. WB: Pig cerebrum and liver tissue lysate. HepG2 cell lysate. Recombinant human GTF2H1 protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 62 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. -
Sequence similarities
Contains 2 BSD domains. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 2965 Human
- Entrez Gene: 14884 Mouse
- Omim: 189972 Human
- SwissProt: P32780 Human
- SwissProt: Q9DBA9 Mouse
- Unigene: 577202 Human
- Unigene: 22700 Mouse
-
Alternative names
- Basic transcription factor 2 62 kDa subunit antibody
- Basic transcription factor 62 kDa subunit antibody
- BTF 2 antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human liver tissue stained for GTF2H1 with ab232982 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + Pig cerebrum tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Anti-GTF2H1 antibody (ab232982) at 5 µg/ml + Pig liver tissue lysate
Secondary
HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/ml
Predicted band size: 62 kDa -
Anti-GTF2H1 antibody (ab232982) at 2 µg/ml + Recombinant human GTF2H1 protein
Predicted band size: 62 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab232982 has been referenced in 1 publication.
- Haase J et al. The TFIIH complex is required to establish and maintain mitotic chromosome structure. Elife 11:N/A (2022). PubMed: 35293859