Product nameAnti-GTF2H1 antibody
See all GTF2H1 primary antibodies
DescriptionRabbit polyclonal to GTF2H1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GTF2H1 aa 250-300. The exact sequence is proprietary. NP_001135779.1
Database link: P32780
- WB: HEK-293T, Jurkat and HeLa whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 62 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
Sequence similaritiesContains 2 BSD domains.
- Information by UniProt
- Basic transcription factor 2 62 kDa subunit antibody
- Basic transcription factor 62 kDa subunit antibody
- BTF 2 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling GTF2H1 with ab272022 at a 1/1,000 dilution. Detection: DAB.
All lanes : Anti-GTF2H1 antibody (ab272022) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Predicted band size: 62 kDa
Exposure time: 3 minutes
GTF2H1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab272022 at 6 μg/mg lysate. WB was performed using ab272022 at 1 µg/ml.
Lane 1: ab272022 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP.
Exposure time: 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272022 has not yet been referenced specifically in any publications.