Overview

  • Product name
    Anti-GTF2H4 antibody [362C2a]
    See all GTF2H4 primary antibodies
  • Description
    Mouse monoclonal [362C2a] to GTF2H4
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GTF2H4 recombinant fragment (Human), from an internal part of the molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab54368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
  • Sequence similarities
    Belongs to the TFB2 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic transcription factor 2 52 kDa subunit antibody
    • Basic transcription factor 52 kDa subunit antibody
    • BTF2 p52 antibody
    • General transcription factor IIH polypeptide 4 52kDa antibody
    • General transcription factor IIH polypeptide 4 52kDa subunit antibody
    • General transcription factor IIH polypeptide 4 52kDa variant antibody
    • General transcription factor IIH polypeptide 4 antibody
    • General transcription factor IIH subunit 4 antibody
    • Gtf2h4 antibody
    • OTTHUMP00000029049 antibody
    • OTTHUMP00000164874 antibody
    • P52 antibody
    • TF2H4_HUMAN antibody
    • TFB2 antibody
    • TFIIH basal transcription factor complex p52 subunit antibody
    • TFIIH protein antibody
    • TIFIIH antibody
    • Transcription factor II H antibody
    • Transcription factor IIH 52 kDa subunit antibody
    see all

Images

  • Anti-GTF2H4 antibody [362C2a] (ab54368) + immunising recombinant fragment

    Predicted band size: 52 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

ab54368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

I would like to reassure you that in the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

As discussed on the telephone, I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also providesome images which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.


Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:



General Information
Antibody storage conditions (temperature/reconstitution etc)


Description of the problem (high background, wrong band size, more bands, no band etc.)


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


Amount of protein loaded


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


Detection method (ECL, ECLPlus etc.)


Positive and negative controls used (please specify)



Optimization attempts (problem solving)
How many times have you tried the Western?



Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?


What steps have you altered?


Additional Notes:


Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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