Key features and details
- Rabbit polyclonal to GTF2IRD1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GTF2IRD1 antibody
See all GTF2IRD1 primary antibodies
DescriptionRabbit polyclonal to GTF2IRD1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Rhesus monkey
Recombinant fragment within Human GTF2IRD1 (internal sequence). The exact sequence is proprietary.
Database link: Q9UHL9
- WB: HeLa whole cell and nuclear extracts. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 106 kDa.|
FunctionMay be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
Tissue specificityHighly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
Involvement in diseaseNote=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Sequence similaritiesBelongs to the TFII-I family.
Contains 5 GTF2I-like repeats.
Developmental stageHighly expressed in developing and regenerating muscles, at the time of myofiber diversification.
DomainThe N-terminal half may have an activating activity.
- Information by UniProt
- CREAM1 antibody
- General transcription factor II I repeat domain containing protein 1 antibody
- General transcription factor II-I repeat domain-containing protein 1 antibody
All lanes : Anti-GTF2IRD1 antibody (ab229718) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 106 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GTF2IRD1 (green) using ab229718 at 1/500 dilution in ICC/IF.
Counterstain: Phalloidin, a cytoskeleton marker, labeled at 1/50 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229718 has not yet been referenced specifically in any publications.