Overview

  • Product name

  • Description

    Rabbit polyclonal to GTF2IRD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human GTF2IRD1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UHL9

  • Positive control

    • WB: HeLa whole cell and nuclear extracts. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 106 kDa.

Target

  • Function

    May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
  • Tissue specificity

    Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
  • Involvement in disease

    Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities

    Belongs to the TFII-I family.
    Contains 5 GTF2I-like repeats.
  • Developmental stage

    Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
  • Domain

    The N-terminal half may have an activating activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CREAM1 antibody
    • General transcription factor II I repeat domain containing protein 1 antibody
    • General transcription factor II-I repeat domain-containing protein 1 antibody
    • General transcription factor III antibody
    • GT2D1_HUMAN antibody
    • GTF2I repeat domain containing protein 1 antibody
    • GTF2I repeat domain-containing protein 1 antibody
    • GTF2IRD1 antibody
    • GTF3 antibody
    • Muscle TFII I repeat domain-containing protein 1 antibody
    • Muscle TFII-I repeat domain-containing protein 1 antibody
    • MUSTRD1 antibody
    • MusTRD1/BEN antibody
    • RBAP2 antibody
    • Slow muscle fiber enhancer binding protein antibody
    • Slow-muscle-fiber enhancer-binding protein antibody
    • USE B1 binding protein antibody
    • USE B1-binding protein antibody
    • WBSCR11 antibody
    • WBSCR12 antibody
    • Williams Beuren syndrome chromosome region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 12 protein antibody
    see all

Images

  • All lanes : Anti-GTF2IRD1 antibody (ab229718) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 106 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GTF2IRD1 (green) using ab229718 at 1/500 dilution in ICC/IF.

    Counterstain: Phalloidin, a cytoskeleton marker, labeled at 1/50 dilution.

References

ab229718 has not yet been referenced specifically in any publications.

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