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    gtf3c3-antibody-ab74230.pdf

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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol III Transcription
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Anti-gtf3c3 antibody (ab74230)

  • Datasheet
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Western blot - Anti-gtf3c3 antibody (ab74230)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)
  • Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)

Key features and details

  • Rabbit polyclonal to gtf3c3
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-gtf3c3 antibody
  • Description

    Rabbit polyclonal to gtf3c3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)

  • Positive control

    • HeLa whole cell lysate (ab150035). Mouse NIH3T3 whole Cell Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol III Transcription

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
All applications
Rat
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
  • Sequence similarities

    Contains 11 TPR repeats.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9Y5Q9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9330 Human
    • Entrez Gene: 98488 Mouse
    • Entrez Gene: 316810 Rat
    • Omim: 604888 Human
    • SwissProt: Q9Y5Q9 Human
    • Unigene: 470996 Human
    • Unigene: 25856 Mouse
    • Unigene: 6721 Rat
    • Alternative names

      • General transcription factor 3C polypeptide 3 antibody
      • general transcription factor IIIC, polypeptide 3 (102kD) antibody
      • general transcription factor IIIC, polypeptide 3, 102kD antibody
      • GTF3C3 antibody
      • TF3C-gamma antibody
      • TF3C3_HUMAN antibody
      • TFIIIC 102 kDa subunit antibody
      • TFIIIC gamma antibody
      • TFIIIC102 antibody
      • TFIIIC2 102 antibody
      • TFIIICgamma antibody
      • Transcription factor IIIC 102 kDa subunit antibody
      • transcription factor IIIC gamma subunit antibody
      • Transcription factor IIIC subunit gamma antibody
      • transcription factor IIIC, 102 kD antibody
      see all

    Images

    • Western blot - Anti-gtf3c3 antibody (ab74230)
      Western blot - Anti-gtf3c3 antibody (ab74230)
      All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T cell at 50 µg
      Lane 5 : mouse NIH3T3 cell at 50 µg

      Predicted band size: 101 kDa
      Observed band size: 101 kDa


      Exposure time: 3 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)
      Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)
      Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
      Lane 2: control IgG.
      Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 3 minutes.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab74230? Please let us know so that we can cite the reference in this datasheet.

    ab74230 has not yet been referenced specifically in any publications.

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