- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-gtf3c3 antibody
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DescriptionRabbit polyclonal to gtf3c3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)
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Positive control
- HeLa whole cell lysate. Mouse NIH3T3 whole Cell Lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab74230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 101 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionInvolved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
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Sequence similaritiesContains 11 TPR repeats.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 9330 Human
- Entrez Gene: 98488 Mouse
- Entrez Gene: 316810 Rat
- Omim: 604888 Human
- SwissProt: Q9Y5Q9 Human
- Unigene: 470996 Human
- Unigene: 25856 Mouse
- Unigene: 6721 Rat
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Alternative names
- General transcription factor 3C polypeptide 3 antibody
- general transcription factor IIIC, polypeptide 3 (102kD) antibody
- general transcription factor IIIC, polypeptide 3, 102kD antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB. -
Western blot - Anti-gtf3c3 antibody (ab74230)All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell at 50 µg
Lane 5 : mouse NIH3T3 cell at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
Lane 2: control IgG.
Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
References
ab74230 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"