Anti-gtf3c3 antibody (ab74230)
Key features and details
- Rabbit polyclonal to gtf3c3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-gtf3c3 antibody -
Description
Rabbit polyclonal to gtf3c3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)
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Positive control
- HeLa whole cell lysate (ab150035). Mouse NIH3T3 whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rat
Rabbit
Horse
Guinea pig
Cow
Dog
Pig
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 101 kDa. |
|
IP
Use at 2-5 µg/mg of lysate. |
|
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters. -
Sequence similarities
Contains 11 TPR repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9330 Human
- Entrez Gene: 98488 Mouse
- Entrez Gene: 316810 Rat
- Omim: 604888 Human
- SwissProt: Q9Y5Q9 Human
- Unigene: 470996 Human
- Unigene: 25856 Mouse
- Unigene: 6721 Rat
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Alternative names
- General transcription factor 3C polypeptide 3 antibody
- general transcription factor IIIC, polypeptide 3 (102kD) antibody
- general transcription factor IIIC, polypeptide 3, 102kD antibody
see all
Images
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Western blot - Anti-gtf3c3 antibody (ab74230)All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell at 50 µg
Lane 5 : mouse NIH3T3 cell at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
Lane 2: control IgG.
Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Protocols
Datasheets and documents
References (0)
ab74230 has not yet been referenced specifically in any publications.
