Product nameAnti-gtf3c3 antibody
DescriptionRabbit polyclonal to gtf3c3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)
- HeLa whole cell lysate (ab150035). Mouse NIH3T3 whole Cell Lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 101 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Sequence similaritiesContains 11 TPR repeats.
- Information by UniProt
- General transcription factor 3C polypeptide 3 antibody
- general transcription factor IIIC, polypeptide 3 (102kD) antibody
- general transcription factor IIIC, polypeptide 3, 102kD antibody
All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell at 50 µg
Lane 5 : mouse NIH3T3 cell at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
Lane 2: control IgG.
Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
ab74230 has not yet been referenced specifically in any publications.