Key features and details
- Rabbit polyclonal to GTF3C4
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GTF3C4 antibody
See all GTF3C4 primary antibodies
DescriptionRabbit polyclonal to GTF3C4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1 and 50 of human GTF3C4 (NP_036336.1)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to GTF3C4/TFIIIC90 immobilized on solid support.
Our Abpromise guarantee covers the use of ab74229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 92 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEssential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Sequence similaritiesBelongs to the TFIIIC subunit 4 family.
- Information by UniProt
- EC 188.8.131.52 antibody
- FLJ21002 antibody
- General transcription factor 3C 4 antibody
All lanes : Anti-GTF3C4 antibody (ab74229) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 185 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
ab74229 has not yet been referenced specifically in any publications.