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Rabbit polyclonal to GTF3C4
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1 and 50 of human GTF3C4 (NP_036336.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Antibody was affinity purified using an epitope specific to GTF3C4/TFIIIC90 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 92 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Belongs to the TFIIIC subunit 4 family.
Information by UniProt
EC 188.8.131.52 antibody
General transcription factor 3C 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF3C4 antibody (ab74229)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-GTF3C4 antibody (ab74229)
All lanes :
Anti-GTF3C4 antibody (ab74229) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
95 kDa (
why is the actual band size different from the predicted?
Additional bands at:
185 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-GTF3C4 antibody (ab74229)
ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2: control IgG.
Detection: Chemiluminescence with an exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"