Product nameAnti-GTF3C5 antibody
See all GTF3C5 primary antibodies
DescriptionRabbit polyclonal to GTF3C5
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide from a region between residue 469 and 519 of human GTF3C5 (using the numbering given in entry NP_036219.1)
- Lysate from HeLa, 293T or NIH3T3 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
Sequence similaritiesBelongs to the TFIIIC subunit 5 family.
- Information by UniProt
- FLJ20857 antibody
- General transcription factor 3C polypeptide 5 antibody
- General transcription factor IIIC polypeptide 5 63kDa antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
ab74305 has not yet been referenced specifically in any publications.