Product nameGTPase Assay Kit
Assay typeEnzyme activity (quantitative)
Assay durationMultiple steps standard assay
GTPase Assay Kit (ab270553) employs a 96-well plate format with all of the reagents necessary for measuring GTPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising out of non-enzymatic GTP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLockTM gives a stable end- point signal and is not prone to precipitation. PiColorLockTM has special additives to prevent backgrounds arising from nonenzymatic GTP hydrolysis.
This product is manufactured by Expedeon, an Abcam company. It was previously called GTPase Enzyme Assay Kit. Expedeon product code 602-0120 is the same as the 2 x 96 tests size of this product.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsPlease refer to protocols.
Components 2 x 96 tests 0.1 M Magnesium chloride 1 x 1.5ml 0.1 mM Pi Standard 1 x 5ml 0.5 M Tris pH 7.5 1 x 5ml Accelerator 1 x 0.25ml 96-well plates 2 units GTP (lyophilized) 4 vials PiColorLock 1 x 10ml Stabilizer 1 x 5ml
Our Abpromise guarantee covers the use of ab270553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Data provided for demonstration purposes only.
Standard Curve for GTPase Assay Kit (ab270553).
Dynamic Range for GTPase Assay Kit.
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.
ab270553 has not yet been referenced specifically in any publications.