Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
Product nameGTPase Assay Kit
Assay typeEnzyme activity (quantitative)
Assay durationMultiple steps standard assay
GTPase Assay Kit (ab270553) employs a 96-well plate format with all of the reagents necessary for measuring GTPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising out of non-enzymatic GTP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLockTM gives a stable end- point signal and is not prone to precipitation. PiColorLockTM has special additives to prevent backgrounds arising from nonenzymatic GTP hydrolysis.
This product is manufactured by Expedeon, an Abcam company. It was previously called GTPase Enzyme Assay Kit. Expedeon product code 602-0120 is the same as the 2 x 96 tests size of this product.
Storage instructionsPlease refer to protocols.
Components 2 x 96 tests ab274109 - 0.1 M Magnesium chloride 1 x 1500µl ab273991 - 0.1 mM Pi Standard 1 x 5ml ab274110 - 0.5 M Tris pH 7.5 1 x 5ml ab274107 - 96-well clear low binding plates 2 units ab273989 - Accelerator 1 x 500µl ab274111 - GTP (lyophilized) 4 vials ab274293 - PiColorLock 1 x 10ml ab273990 - Stabilizer 1 x 5ml
Data provided for demonstration purposes only.
Standard Curve for GTPase Assay Kit (ab270553).
Dynamic Range for GTPase Assay Kit.
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.
ab270553 has not yet been referenced specifically in any publications.