Overview

  • Product name
    Anti-GTPase HRAS antibody [Y132]
    See all GTPase HRAS primary antibodies
  • Description
    Rabbit monoclonal [Y132] to GTPase HRAS
  • Host species
    Rabbit
  • Specificity
    Reactivity with other RAS members has not been tested.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide within Human GTPase HRAS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control
    • MCF7 and PC12 cell lysates and MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa.
IP 1/50 - 1/60.
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function
      Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • Involvement in disease
      Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • Sequence similarities
      Belongs to the small GTPase superfamily. Ras family.
    • Post-translational
      modifications
      Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • Cellular localization
      Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C BAS/HAS antibody
      • c H ras antibody
      • C HA RAS1 antibody
      • c has/bas p21 protein antibody
      • c ras Ki 2 activated oncogene antibody
      • c-H-ras antibody
      • CTLO antibody
      • GTP and GDP binding peptide B antibody
      • GTPase HRas, N-terminally processed antibody
      • H Ras 1 antibody
      • H RASIDX antibody
      • H-Ras-1 antibody
      • Ha Ras antibody
      • Ha Ras1 proto oncoprotein antibody
      • Ha-Ras antibody
      • HAMSV antibody
      • Harvey rat sarcoma viral oncogene homolog antibody
      • Harvey rat sarcoma viral oncoprotein antibody
      • HRAS antibody
      • HRAS1 antibody
      • K ras antibody
      • N ras antibody
      • p19 H RasIDX protein antibody
      • p21ras antibody
      • Ras family small GTP binding protein H Ras antibody
      • RASH_HUMAN antibody
      • RASH1 antibody
      • Transformation gene oncogene HAMSV antibody
      • Transforming protein p21 antibody
      • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
      • VH Ras antibody
      • vHa RAS antibody
      see all

    Images

    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/2500 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size: 21 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/1000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab32417 (purified) at 1/60 immunoprecipitating GTPase in 10 μg mouse brain whole cell lysate (Lanes 1 and 2, observed at 21 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate

      Observed band size: 21 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    See all 8 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for contacting us.
    ab97488 and ab6548 are alternative to sc-520. Please note this assumption is based on the location of immunogen only. We might not have similar species or application data.
    ab140962 and ab140377 are expected alternatives for sc29.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you for your reply. I have created the discount code below, which can be redeemed for a free primary antibody after submitting a review of ab96548 and following the instructions below. Please let me know if you have any questions or if there is anything else that I can help you with.
    DISCOUNT CODE: *** Expiration date: June 16, 2012
    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab96548 with mouse samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting us with your enquiry.
    I have run some alignments between the immunogens of ab64705 and ab96548, and ab96548 is more specific for HRas. The immunogen of ab64705 overlaps quite a bit with N and KRas, however ab96548 shows no significant similarity to KRas and very little (47%) to NRas.
    We only have the full size vials of all of our products and are not able to send trial sizes. However, as ab96548 has not been tested in murine samples, you are eligible for our testing discount program if you would like to try this antibody in mouse. For this program, you would initially purchase ab96548, test it in mouse, then submit an Abreview with your data. In exchange we will give you a discount code valid for one free primary antibody on a future order.
    I hope this information will be useful. Please let me know if you have any questions, or if you would like to participate in our testing discount program, and I will be happy to arrange that for you.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Colon cancer cells)
    Loading amount
    100 µg
    Specification
    Colon cancer cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Oct 17 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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