For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    gtpase-hras-antibody-y132-ab32417.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Associated Proteins
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GTPase HRAS antibody [Y132] (ab32417)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (13)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
  • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
  • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
  • Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)
  • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
  • Anti-GTPase HRAS antibody [Y132] (ab32417)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y132] to GTPase HRAS
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Biochemical
Product image
GTP-gamma-S, G-protein-activating GTP analog (ab146662)
Primary
Product image
Anti-Ras antibody [EPR18713-13] (ab206969)
Primary
Product image
Anti-Ras antibody [EPR3255] - BSA and Azide free (ab209974)

View more associated products

Overview

  • Product name

    Anti-GTPase HRAS antibody [Y132]
    See all GTPase HRAS primary antibodies
  • Description

    Rabbit monoclonal [Y132] to GTPase HRAS
  • Host species

    Rabbit
  • Specificity

    Reactivity with other RAS members has not been tested.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide within Human GTPase HRAS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • MCF7 and PC12 cell lysates and MCF7 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y132
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • GTP
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Alternative Versions

    • Anti-GTPase HRAS antibody [Y132] - BSA and Azide free (ab239815)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human HRAS knockout HEK-293 cell line (ab260858)
  • Recombinant Protein

    • Recombinant Human GTPase HRAS protein (ab93949)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/1000. Detects a band of approximately 21 kDa.
IP
1/50 - 1/60.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 21 kDa.
IP
1/50 - 1/60.
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function

      Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
    • Involvement in disease

      Defects in HRAS are the cause of faciocutaneoskeletal syndrome (FCSS) [MIM:218040]. A rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities.
      Defects in HRAS are the cause of congenital myopathy with excess of muscle spindles (CMEMS) [MIM:218040]. CMEMS is a variant of Costello syndrome.
      Defects in HRAS may be a cause of susceptibility to Hurthle cell thyroid carcinoma (HCTC) [MIM:607464]. Hurthle cell thyroid carcinoma accounts for approximately 3% of all thyroid cancers. Although they are classified as variants of follicular neoplasms, they are more often multifocal and somewhat more aggressive and are less likely to take up iodine than are other follicular neoplasms.
      Note=Mutations which change positions 12, 13 or 61 activate the potential of HRAS to transform cultured cells and are implicated in a variety of human tumors.
      Defects in HRAS are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.
      Note=Defects in HRAS are the cause of oral squamous cell carcinoma (OSCC).
    • Sequence similarities

      Belongs to the small GTPase superfamily. Ras family.
    • Post-translational
      modifications

      Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      S-nitrosylated; critical for redox regulation. Important for stimulating guanine nucleotide exchange. No structural perturbation on nitrosylation.
    • Cellular localization

      Cell membrane. Golgi apparatus membrane. The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
    • Target information above from: UniProt accession P01112 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396229 Chicken
      • Entrez Gene: 3265 Human
      • Entrez Gene: 15461 Mouse
      • Entrez Gene: 293621 Rat
      • Omim: 190020 Human
      • SwissProt: P08642 Chicken
      • SwissProt: P01112 Human
      • SwissProt: Q61411 Mouse
      • SwissProt: P20171 Rat
      • Unigene: 37003 Human
      • Unigene: 334313 Mouse
      • Unigene: 102180 Rat
      see all
    • Alternative names

      • C BAS/HAS antibody
      • c H ras antibody
      • C HA RAS1 antibody
      • c has/bas p21 protein antibody
      • c ras Ki 2 activated oncogene antibody
      • c-H-ras antibody
      • CTLO antibody
      • GTP and GDP binding peptide B antibody
      • GTPase HRas, N-terminally processed antibody
      • H Ras 1 antibody
      • H RASIDX antibody
      • H-Ras-1 antibody
      • Ha Ras antibody
      • Ha Ras1 proto oncoprotein antibody
      • Ha-Ras antibody
      • HAMSV antibody
      • Harvey rat sarcoma viral oncogene homolog antibody
      • Harvey rat sarcoma viral oncoprotein antibody
      • HRAS antibody
      • HRAS1 antibody
      • K ras antibody
      • N ras antibody
      • p19 H RasIDX protein antibody
      • p21ras antibody
      • Ras family small GTP binding protein H Ras antibody
      • RASH_HUMAN antibody
      • RASH1 antibody
      • Transformation gene oncogene HAMSV antibody
      • Transforming protein p21 antibody
      • v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
      • VH Ras antibody
      • vHa RAS antibody
      see all

    Images

    • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution

      Lane 1 : Wild-type HEK-293 whole cell lysate
      Lane 2 : HRAS knockout HEK-293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg/ml per lane.


      Lanes 1 - 4: Merged signal (red and green). Green - ab32417 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32417 was shown to recognize HRAS in wild-type HEK-293 cells as signal was lost at the expected MW in HRAS knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and HRAS knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab32417 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/2500 dilution (purified)

      Lane 1 : mouse brain lysate
      Lane 2 : rat brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/1000 dilution (purified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Observed band size: 21 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)
      Immunoprecipitation - Anti-GTPase HRAS antibody [Y132] (ab32417)

      ab32417 (purified) at 1/60 immunoprecipitating GTPase in 10 μg mouse brain whole cell lysate (Lanes 1 and 2, observed at 21 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      Western blot - Anti-GTPase HRAS antibody [Y132] (ab32417)
      All lanes : Anti-GTPase HRAS antibody [Y132] (ab32417) at 1/500 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : PC12 cell lysate

      Observed band size: 21 kDa why is the actual band size different from the predicted?

    • Anti-GTPase HRAS antibody [Y132] (ab32417)
      Anti-GTPase HRAS antibody [Y132] (ab32417)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab32417? Please let us know so that we can cite the reference in this datasheet.

    ab32417 has been referenced in 13 publications.

    • Liu J  et al. Ethanol Extract of the Infructescence of Platycarya strobilacea Sieb. et Zucc. Induces Methuosis of Human Nasopharyngeal Carcinoma Cells. Evid Based Complement Alternat Med 2020:2760979 (2020). PubMed: 32419796
    • Zhao T  et al. DNA methylation-regulated QPCT promotes sunitinib resistance by increasing HRAS stability in renal cell carcinoma. Theranostics 9:6175-6190 (2019). PubMed: 31534544
    • Anta B  et al. PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes. Cell Death Dis 7:e2311 (2016). WB . PubMed: 27468687
    • Yokoi N  et al. Identification of PSD-95 Depalmitoylating Enzymes. J Neurosci 36:6431-44 (2016). PubMed: 27307232
    • Kong G  et al. Loss of wild-type Kras promotes activation of all Ras isoforms in oncogenic Kras-induced leukemogenesis. Leukemia 30:1542-51 (2016). PubMed: 27055865
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Western blot abreview for Anti-GTPase HRAS antibody [Y132]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Colon cancer cells)
    Loading amount
    100 µg
    Specification
    Colon cancer cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 17 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.