Overview

  • Product name

  • Description

    Rabbit polyclonal to GTPBP4/NOG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human GTPBP4/NOG1 aa 339-613.
    Database link: Q9BZE4

  • Positive control

    • 293T, A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; A431 cells.
  • General notes

     This product was previously labelled as GTPBP4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab153977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 74 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the biogenesis of the 60S ribosomal subunit.
  • Sequence similarities

    Belongs to the GTP1/OBG family. NOG subfamily.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chronic renal failure gene protein antibody
    • CRFG antibody
    • G protein binding protein CRFG antibody
    • GTP binding protein 4 antibody
    • GTP-binding protein NGB antibody
    • Gtpbp4 antibody
    • NGB antibody
    • NOG1 antibody
    • NOG1_HUMAN antibody
    • Nucleolar GTP binding protein 1 antibody
    • Nucleolar GTP-binding protein 1 antibody
    see all

Images

  • All lanes : Anti-GTPBP4/NOG1 antibody (ab153977) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • All lanes : Anti-GTPBP4/NOG1 antibody (ab153977) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of A431 cells (fixed in iced-cold MeOH for 5 min) labeling GTPBP4/NOG1 with ab153977 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342 (blue).

References

ab153977 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153977.
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