Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3500] to GTPBP4/NOG1
- Suitable for: WB, IP, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-GTPBP4/NOG1 antibody [EPR3500]
See all GTPBP4/NOG1 primary antibodies
DescriptionRabbit monoclonal [EPR3500] to GTPBP4/NOG1
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GTPBP4/NOG1 aa 50-150. The exact sequence is proprietary.
- Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as GTPBP4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the biogenesis of the 60S ribosomal subunit.
Sequence similaritiesBelongs to the GTP1/OBG family. NOG subfamily.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Chronic renal failure gene protein antibody
- CRFG antibody
- G protein binding protein CRFG antibody
All lanes : Anti-GTPBP4/NOG1 antibody [EPR3500] (ab92342) at 1/2000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab92342 has been referenced in 2 publications.