Product nameAnti-GTPBP4/NOG1 antibody [EPR3500]
See all GTPBP4/NOG1 primary antibodies
DescriptionRabbit monoclonal [EPR3500] to GTPBP4/NOG1
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GTPBP4/NOG1 aa 50-150. The exact sequence is proprietary.
- Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as GTPBP4
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the biogenesis of the 60S ribosomal subunit.
Sequence similaritiesBelongs to the GTP1/OBG family. NOG subfamily.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- Chronic renal failure gene protein antibody
- CRFG antibody
- G protein binding protein CRFG antibody
All lanes : Anti-GTPBP4/NOG1 antibody [EPR3500] (ab92342) at 1/2000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.