Overview

  • Product name

    Anti-GTPBP4/NOG1 antibody [EPR3500]
    See all GTPBP4/NOG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3500] to GTPBP4/NOG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GTPBP4/NOG1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as GTPBP4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
Flow Cyt
  • Application notes
    Flow Cyt: 1/50.
    IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
    IP: 1/50.
    WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Involved in the biogenesis of the 60S ribosomal subunit.
    • Sequence similarities

      Belongs to the GTP1/OBG family. NOG subfamily.
    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Chronic renal failure gene protein antibody
      • CRFG antibody
      • G protein binding protein CRFG antibody
      • GTP binding protein 4 antibody
      • GTP-binding protein NGB antibody
      • Gtpbp4 antibody
      • NGB antibody
      • NOG1 antibody
      • NOG1_HUMAN antibody
      • Nucleolar GTP binding protein 1 antibody
      • Nucleolar GTP-binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-GTPBP4/NOG1 antibody [EPR3500] (ab92342) at 1/2000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Human placenta tissue lysate
      Lane 4 : Human testis tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 74 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • ab92342 at 1/250 dilution staining GTPBP4/NOG1 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab92342.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up