Product nameGuanidine hydrochloride, Chaotropic agent
DescriptionStrong chaotropic agent and protein denaturant
- Aminoformamidine hydrochloride
- Carbamimidoylazanium chloride
- Chloride, Guanidinium
Normal product of protein metabolism. Strong chaotropic agent and protein denaturant widely used for purification of proteins and nucleic acids. Solubilizes insoluble or denatured proteins such as inclusion bodies and can be used as the first step in refolding proteins or enzymes into their active form. Used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Chemical nameGuanidine hydrochloride
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM, in water to 100 mM and in ethanol to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Jung G & Masison DC Guanidine hydrochloride inhibits Hsp104 activity in vivo: a possible explanation for its effect in curing yeast prions. Curr Microbiol 43:7-10 (2001). Read more (PubMed: 11375656) »
- Hagihara Y et al. Guanidine hydrochloride-induced folding of proteins. J Mol Biol 231:180-4 (1993). Read more (PubMed: 8389881) »
- Pace CN et al. pH dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1. Biochemistry 29:2564-72 (1990). Read more (PubMed: 2110472) »
- Pace CN Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol 131:266-80 (1986). Read more (PubMed: 3773761) »
- Greene RF & Pace CN Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, alpha-chymotrypsin, and beta-lactoglobulin. J Biol Chem 249:5388-93 (1974). Read more (PubMed: 4416801) »