Guanidine hydrochloride, Chaotropic agent (ab145746)

Overview

  • Product name

    Guanidine hydrochloride, Chaotropic agent
  • Description

    Strong chaotropic agent and protein denaturant
  • Alternative names

    • Aminoformamidine hydrochloride
    • Carbamimidoylazanium chloride
    • Chloride, Guanidinium
    • Chloride, Guanidium
    • GdmCl
    • GndC
    • Guanidine HCl
    • Guanidinium chloride
    • GuHCl
    see all
  • Biological description

    Normal product of protein metabolism. Strong chaotropic agent and protein denaturant widely used for purification of proteins and nucleic acids. Solubilizes insoluble or denatured proteins such as inclusion bodies and can be used as the first step in refolding proteins or enzymes into their active form. Used in RNA isolation to dissociate nucleoproteins and inhibit RNase.

  • CAS Number

    50-01-1
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    Guanidine hydrochloride
  • Molecular weight

    95.53
  • Molecular formula

    CH5N3.HCl
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO to 100 mM, in water to 100 mM and in ethanol to 50 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Jung G & Masison DC Guanidine hydrochloride inhibits Hsp104 activity in vivo: a possible explanation for its effect in curing yeast prions. Curr Microbiol 43:7-10 (2001). Read more (PubMed: 11375656) »
  • Hagihara Y  et al. Guanidine hydrochloride-induced folding of proteins. J Mol Biol 231:180-4 (1993). Read more (PubMed: 8389881) »
  • Pace CN  et al. pH dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1. Biochemistry 29:2564-72 (1990). Read more (PubMed: 2110472) »
  • Pace CN Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol 131:266-80 (1986). Read more (PubMed: 3773761) »
  • Greene RF & Pace CN Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, alpha-chymotrypsin, and beta-lactoglobulin. J Biol Chem 249:5388-93 (1974). Read more (PubMed: 4416801) »

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