Key features and details
- Rabbit polyclonal to Guanylate Cyclase
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Guanylate Cyclase antibody
See all Guanylate Cyclase primary antibodies
DescriptionRabbit polyclonal to Guanylate Cyclase
SpecificityThis antibody detects endogenous levels of total Guanylate Cyclase ß protein.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from Human Guanylate Cyclase ß
- HuvEc cell extract; Paraffin-embedded human lung carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||Use at an assay dependent concentration.|
RelevanceGuanylate Cyclase is an heterodimer of an alpha and a beta chain. Its enzymatic activity transforms GTP to GMP releasing a diphosphate. It binds 1 or 2 hemes per heterodimer. It is activated by nitric oxide in the presence of magnesium or manganese ions. There are two types of guanylate cyclase: soluble form and membrane-associated receptor form.
- GC SB2 antibody
- GCS beta 2 antibody
- Guanylate cyclase 1 soluble beta 2 antibody
- GUCY1B2 antibody
All lanes : Anti-Guanylate Cyclase antibody (ab53084) at 1/300 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with peptide
Predicted band size: 70 kDa
Observed band size: 70 kDa
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Guanylate Cyclase ß antibody, ab53084, at 1/50 dilution.
ab53084 has been referenced in 1 publication.
- Wu RY & Ma N Expression of nitric oxide synthase and guanylate cyclase in the human ciliary body and trabecular meshwork. Chin Med J (Engl) 125:129-33 (2012). WB, IHC-P ; Human . PubMed: 22340479