Key features and details
- Rabbit polyclonal to Guanylin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Guanylin antibody
See all Guanylin primary antibodies
DescriptionRabbit polyclonal to Guanylin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human small intestine tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionEndogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.
Tissue specificityHighly expressed in ileum and colon. Found in plasma.
Sequence similaritiesBelongs to the guanylin family.
- Information by UniProt
- GapI antibody
- GCAP-I antibody
- Guanylate cyclase activating protein 1 antibody
Paraffin embedded human small intestine tissue stained for Guanylin with ab223727 (1/1000 dilution) in immunohistochemical analysis.
All lanes : Anti-Guanylin antibody (ab223727) at 1/100 dilution
Lane 1 : Control (vector only transfected HEK293T lysate)
Lane 2 : Guanylin over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK-293T cells
Predicted band size: 12 kDa
ab223727 has not yet been referenced specifically in any publications.