• Product name

    Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody
    See all Guanylyl Cyclase alpha 1/GUCY1A3 primary antibodies
  • Description

    Rabbit polyclonal to Guanylyl Cyclase alpha 1/GUCY1A3
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Rat Guanylyl Cyclase alpha 1/GUCY1A3 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab97987)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Rat kidney; Rat lung; Mouse lung; Rat heart. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
  • General notes

     This product was previously labelled as Guanylyl Cyclase alpha 1




Our Abpromise guarantee covers the use of ab85445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).


  • Sequence similarities

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • GC S alpha 1 antibody
    • GC SA3 antibody
    • GCS alpha 1 antibody
    • GCS alpha 3 antibody
    • GCS-alpha-1 antibody
    • GCS-alpha-3 antibody
    • GCYA3_HUMAN antibody
    • Guanylate cyclase 1 soluble alpha 3 antibody
    • Guanylate cyclase soluble subunit alpha 1 antibody
    • Guanylate cyclase soluble subunit alpha 3 antibody
    • Guanylate cyclase soluble subunit alpha-3 antibody
    • Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit antibody
    • Guc1a1 antibody
    • GUC1A3 antibody
    • GUCA3 antibody
    • GUCSA3 antibody
    • Gucy1a1 antibody
    • Gucy1a3 antibody
    • MYMY6 antibody
    • nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit antibody
    • NOGC, alpha-1 subunit antibody
    • Soluble guanylate cyclase large subunit antibody
    see all


  • All lanes : Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445) at 1 µg/ml

    Lane 1 : Kidney (Rat) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 77 kDa
    Observed band size: 77 kDa
    Additional bands at: 48 kDa, 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • IHC image of Guanylyl Cyclase alpha 1/GUCY1A3 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  • ICC/IF image of ab85445 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85445 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab85445 has not yet been referenced specifically in any publications.

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