Product nameAnti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody
See all Guanylyl Cyclase alpha 1/GUCY1A3 primary antibodies
DescriptionRabbit polyclonal to Guanylyl Cyclase alpha 1/GUCY1A3
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide corresponding to Rat Guanylyl Cyclase alpha 1/GUCY1A3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Rat kidney; Rat lung; Mouse lung; Rat heart. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
This product was previously labelled as Guanylyl Cyclase alpha 1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).|
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
- Information by UniProt
- GC S alpha 1 antibody
- GC SA3 antibody
- GCS alpha 1 antibody
All lanes : Anti-Guanylyl Cyclase alpha 1/GUCY1A3 antibody (ab85445) at 1 µg/ml
Lane 1 : Kidney (Rat) Tissue Lysate
Lane 2 : Lung (Rat) Tissue Lysate
Lane 3 :
Mouse lung normal tissue lysate - total protein (ab29297)
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Additional bands at: 48 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
IHC image of Guanylyl Cyclase alpha 1/GUCY1A3 staining in human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85445, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
ICC/IF image of ab85445 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85445 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab85445 has not yet been referenced specifically in any publications.