Product nameAnti-Guanylyl Cyclase beta 1 antibody
See all Guanylyl Cyclase beta 1 primary antibodies
DescriptionRabbit polyclonal to Guanylyl Cyclase beta 1
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Dog, Pig
Synthetic peptide corresponding to Guanylyl Cyclase beta 1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab24824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
Contains 1 guanylate cyclase domain.
- Information by UniProt
- GC-S-beta-1 antibody
- GC-SB3 antibody
- GCbeta1 antibody
Human cerebellum stained with Anti Guanylyl cyclase beta 1 ab24824 at 1/100 dilution for 30 min at RT.
ICC/IF image of ab24824 stained SKNSH cells. The cells were Both and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24824, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Tsihlis ND et al. Nitric oxide is less effective at inhibiting neointimal hyperplasia in spontaneously hypertensive rats. Nitric Oxide 35:165-74 (2013). IHC-P ; Rat . Read more (PubMed: 24149190) »
- van Heerebeek L et al. Low myocardial protein kinase g activity in heart failure with preserved ejection fraction. Circulation 126:830-9 (2012). IHC-P ; Human . Read more (PubMed: 22806632) »