Overview

  • Product name

    Anti-GUCY1B3 antibody [EPR8822]
    See all GUCY1B3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8822] to GUCY1B3
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GUCY1B3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human GUCY1B3 protein (ab152446) can be used as a positive control in WB. Human placenta lysate; Human fetal brain and fetal lung lysates; Human pancreas tissue; Human thyroid gland carcinoma tissue; Mouse brain; Rat brain; Rat spleen; Human kidney.
  • General notes

     This product was previously labelled as Guanylyl Cyclase beta 1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8822
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 -1/250. 

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling GUCY1B3 with Purified ab154841 at 1:400 dilution (2.54 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/5000 dilution (purified)

    Lane 1 : Human fetal lung lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates
    Lane 4 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 71 kDa



    Blocking and diluting buffer : 5% NFDM/TBST
  • All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal lung lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 71 kDa

  • All lanes : Anti-GUCY1B3 antibody [EPR8822] (ab154841) at 1/2000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid gland carcinoma tissue, labeling GUCY1B3 using unpurified ab154841 at a 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using unpurified ab154841 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human heart tissue using unpurified ab154841 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using unpurified ab154841 showing +ve staining.

References

This product has been referenced in:

  • Mukohda M  et al. RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity. J Clin Invest 130:2318-2332 (2019). Read more (PubMed: 30896450) »
  • Wales JA  et al. Discovery of stimulator binding to a conserved pocket in the heme domain of soluble guanylyl cyclase. J Biol Chem 293:1850-1864 (2018). Read more (PubMed: 29222330) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brainstem, cochlear nuclei)
Specification
brainstem, cochlear nuclei

Lin Lin

Verified customer

Submitted Mar 31 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung, liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
lung, liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Mar 07 2016

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