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Immunology Innate Immunity Complement Classical Pathway
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Guinea Pig Complement C3 ELISA Kit (ab157705)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (1)References (2)

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Standard Curve

    Key features and details

    • Sensitivity: 1.3083 ng/ml
    • Range: 3.13 ng/ml - 3260 ng/ml
    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Guinea pig

    Overview

    • Product name

      Guinea Pig Complement C3 ELISA Kit
      See all C3 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      1.3083 ng/ml
    • Range

      3.13 ng/ml - 3260 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Guinea pig
    • Product overview

      Abcam's Complement C3 Guinea Pig ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Complement C3 levels in serum and blood . 

      In this assay the Complement C3 present in samples reacts with the anti-Complement C3 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Complement C3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Complement C3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Complement C3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Complement C3 in the test sample. The quantity of Complement C3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X HRP-conjugated anti-guinea pig Complement C3 antibody 1 x 150µl
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chromogen Substrate Solution 1 x 12ml
      Guinea Pig Complement C3 Calibrator (Lyophilized) 1 vial
      Guinea Pig Complement C3 ELISA Microplate 1 unit
      Stop Solution 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Immunology
      • Innate Immunity
      • Complement
      • Alternative Pathway
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Complement ELISA kits
    • Function

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01024 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Acylation stimulating protein cleavage product
      • AHUS5
      • ARMD9
      • ASP
      • C3
      • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      • c3 complement
      • C3adesArg
      • CO3_HUMAN
      • Complement C3
      • Complement C3 alpha chain
      • Complement C3b alpha' chain
      • Complement C3c alpha' chain fragment 1
      • Complement C3c alpha' chain fragment 2
      • Complement C3c alpha'' chain fragment 2
      • Complement C3d fragment
      • Complement C3dg fragment
      • Complement C3f fragment
      • Complement C3g fragment
      • Complement component 3
      • Complement factor 3
      • CPAMD1
      • HEL S 62p
      • omplement C3 beta chain
      see all

    Images

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157705 Complement C3 GuineaPig ELISA Kit.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab157705? Please let us know so that we can cite the reference in this datasheet.

    ab157705 has been referenced in 2 publications.

    • Radakovich LB  et al. Calorie restriction with regular chow, but not a high-fat diet, delays onset of spontaneous osteoarthritis in the Hartley guinea pig model. Arthritis Res Ther 21:145 (2019). PubMed: 31196172
    • Hickman DA  et al. Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage. Sci Rep 8:3118 (2018). PubMed: 29449604

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    The protocol notes that once diluted some of the reagents can only be stored for a week at 4 deg C. Alternatively, can these prepared reagents be stored in aliquots at -20 or -80 deg C for longer periods.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2014

    Answer

    The only components that can be stored for a week at a 1X working dilution are the running diluent and the wash buffer. I would not recommend freezing either.
    No other component in this kit can be stored longer than 8 hours at the 1X working solution.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Jul 02 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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