Product nameGuinea Pig IgG ELISA Kit
See all IgG kits
Intra-assay Sample n Mean SD CV% Serum < 10% Inter-assay Sample n Mean SD CV% Serum < 10%
Sensitivity= 1.069 ng/ml
Range6.25 ng/ml - 400 ng/ml
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 6.25ng/ml - 400ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Guinea pig
Abcam’s IgG Guinea pig ELISA Kit (ab190525) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in serum.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromo¬genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Tested applicationsSuitable for: Sandwich ELISAmore details
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml IgG Guinea Pig Antibody Coated Microplate 1 x 96 tests IgG Guinea Pig Calibrator (lyophilized) 1 vial IgG Guinea Pig HRP Conjugate 1 vial Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
Our Abpromise guarantee covers the use of ab190525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab190525 has not yet been referenced specifically in any publications.