Anti-GULP antibody (ab236893)
Key features and details
- Rabbit polyclonal to GULP
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GULP antibody
See all GULP primary antibodies -
Description
Rabbit polyclonal to GULP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human GULP aa 150-300.
Database link: Q9UBP9 -
Positive control
- WB: HepG2 whole cell lysate; Rat heart lysate. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/500.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 34 kDa.
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Notes |
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ICC/IF
1/50 - 1/500. |
WB
1/2000 - 1/10000. Predicted molecular weight: 34 kDa. |
Target
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Function
May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6. -
Tissue specificity
Widely expressed. Detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary. -
Sequence similarities
Belongs to the ced-6 family.
Contains 1 PID domain. -
Cellular localization
Cytoplasm. May associate with the cytoplasmic side of the plasma membrane and early endosomes. - Information by UniProt
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Database links
- Entrez Gene: 51454 Human
- Entrez Gene: 314543 Rat
- Omim: 608165 Human
- SwissProt: Q9UBP9 Human
- SwissProt: Q5PQS4 Rat
- Unigene: 470887 Human
- Unigene: 712910 Human
- Unigene: 32649 Rat
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Alternative names
- CED6 antibody
- Cell death protein 6 homolog antibody
- Engulfment adapter protein antibody
see all
Images
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All lanes : Anti-GULP antibody (ab236893) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Rat heart tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling GULP using ab236893 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236893 has been referenced in 1 publication.
- McCormick R et al. Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration. Front Bioeng Biotechnol 8:456 (2020). PubMed: 32500067