Key features and details
- Rabbit polyclonal to GULP
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-GULP antibody
See all GULP primary antibodies
DescriptionRabbit polyclonal to GULP
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
- WB: HepG2 whole cell lysate; Rat heart lysate. ICC/IF: HepG2 cells.
Previously labelled as CED6.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/500.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 34 kDa.|
FunctionMay function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6.
Tissue specificityWidely expressed. Detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary.
Sequence similaritiesBelongs to the ced-6 family.
Contains 1 PID domain.
Cellular localizationCytoplasm. May associate with the cytoplasmic side of the plasma membrane and early endosomes.
- Information by UniProt
- CED6 antibody
- Cell death protein 6 homolog antibody
- Engulfment adapter protein antibody
All lanes : Anti-GULP antibody (ab236893) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Rat heart tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling GULP using ab236893 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236893 has not yet been referenced specifically in any publications.