Overview

  • Product name

  • Description

    Goat polyclonal to GULP
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human GULP aa 291-304.
    Sequence:

    EGTVFCLDPLDSRC


    (Peptide available as ab22934)

  • General notes

    Previously labelled as CED6. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    Tested in peptide ELISA at 1:64000.
    IHC-P: Use at a concentration of 5 µg/ml.
    Western Blot: Preliminary experiments gave an approx 35kDa band in K562 lysates at 1µg/ml. However, this was accompanied by stronger bands at approx 45kDa and 48kDa. Please note that currently we cannot find an explanation in the literature for these extra bands given the predicted size of approx. 35kDda according to NP_057399.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May function as an adapter protein. Required for efficient phagocytosis of apoptotic cells. Modulates cellular glycosphingolipid and cholesterol transport. May play a role in the internalization and endosomal trafficking of various LRP1 ligands, such as PSAP. Increases cellular levels of GTP-bound ARF6.
    • Tissue specificity

      Widely expressed. Detected in macrophages, pancreas, kidney, skeletal muscle, heart, colon, intestine, lung, placenta and ovary.
    • Sequence similarities

      Belongs to the ced-6 family.
      Contains 1 PID domain.
    • Cellular localization

      Cytoplasm. May associate with the cytoplasmic side of the plasma membrane and early endosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • CED6 antibody
      • Cell death protein 6 homolog antibody
      • Engulfment adapter protein antibody
      • Engulfment adaptor PTB domain containing 1 antibody
      • GULP antibody
      • GULP, engulfment adaptor PTB domain containing 1 antibody
      • GULP1 antibody
      • GULP1_HUMAN antibody
      • Protein GULP antibody
      • PTB domain adapter protein CED 6 antibody
      • PTB domain adapter protein CED-6 antibody
      • PTB domain adaptor protein CED6 antibody
      • PTB domain-containing engulfment adapter protein 1 antibody
      see all

    Images

    • ab2751 at 5ug/ml staining GULP in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

    References

    This product has been referenced in:

    • Bae DJ  et al. ArhGAP12 plays dual roles in Stabilin-2 mediated efferocytosis: Regulates Rac1 basal activity and spatiotemporally turns off the Rac1 to orchestrate phagosome maturation. Biochim Biophys Acta Mol Cell Res 1866:1595-1607 (2019). Read more (PubMed: 31301364) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab2751.
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