Key features and details
- Rabbit polyclonal to GW182
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GW182 antibody
See all GW182 primary antibodies
DescriptionRabbit polyclonal to GW182
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human GW182 aa 1100-1200 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in HeLa cell line.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab110233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionPlays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
Sequence similaritiesBelongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain.
Cellular localizationCytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
- Information by UniProt
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ICC/IF image of ab110233 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 cells at 1µg/ml.
ab110233 has been referenced in 1 publication.
- Gallego-Iradi MC et al. Subcellular Localization of Matrin 3 Containing Mutations Associated with ALS and Distal Myopathy. PLoS One 10:e0142144 (2015). PubMed: 26528920