Anti-GW182 antibody (ab114857)

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Overview

  • Product name

    Anti-GW182 antibody
    See all GW182 primary antibodies

  • Description

    Rabbit polyclonal to GW182

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1225-1275 of Human GW182 (NP_055309.2).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab114857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the GW182 family.
      Contains 1 RRM (RNA recognition motif) domain.

    • Cellular localization

      Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
    • Information by UniProt

    • Database links

      • Alternative names

        • CAG repeat protein 26 antibody
        • CAGH26 antibody
        • DKFZp666E117 antibody
        • EDIE antibody
        • EMSY interactor protein antibody
        • FLJ22043 antibody
        • Glycine tryptophan protein of 182 kDa antibody
        • Glycine-tryptophan protein of 182 kDa antibody
        • GW1 antibody
        • GW182 antibody
        • GW182 autoantigen antibody
        • Hypothetical protein DKFZp566M143 antibody
        • KIAA1460 antibody
        • MGC75384 antibody
        • OTTHUMP00000122485 antibody
        • OTTHUMP00000195164 antibody
        • OTTHUMP00000195165 antibody
        • Protein GW1 antibody
        • TNR6A_HUMAN antibody
        • TNRC 6A antibody
        • TNRC6 antibody
        • Tnrc6a antibody
        • Trinucleotide repeat containing 6A antibody
        • Trinucleotide repeat containing gene 6A protein antibody
        • Trinucleotide repeat-containing gene 6A protein antibody
        see all

      Images

      • Immunoprecipitation - Anti-GW182 antibody (ab114857)
        Immunoprecipitation - Anti-GW182 antibody (ab114857)
        Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      References

      ab114857 has not yet been referenced specifically in any publications.

      Publishing research using ab114857? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Please arrange a discount code for ab87541. Thank you.  

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      Answer

      DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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      Question

      P {margin-top:2px;margin-bottom:2px;} Dear technical team,   Our customer would like to test ICC/IF with human sample.   She searched ab70522 but, now it's not available so,   Please recommend one of your GW182 antibody to our customer.   She would like to get a discount code for it.   I look forward to hearing from you.   Best regards,

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      Answer

      Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of these products are eligible for Abreview discount programme. Let me know the product you would be interested in using I will then send you the discount code. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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