Anti-GW182 antibody (ab114857)
Key features and details
- Rabbit polyclonal to GW182
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GW182 antibody
See all GW182 primary antibodies -
Description
Rabbit polyclonal to GW182 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1225-1275 of Human GW182 (NP_055309.2).
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Positive control
- HeLa whole cell lysate (ab150035)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab114857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | Use at 10 µg/mg of lysate. |
Target
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Function
Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the GW182 family.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies. - Information by UniProt
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Database links
- Entrez Gene: 27327 Human
- Entrez Gene: 233833 Mouse
- Entrez Gene: 308971 Rat
- Omim: 610739 Human
- SwissProt: Q8NDV7 Human
- SwissProt: Q3UHK8 Mouse
- Unigene: 655057 Human
- Unigene: 102305 Mouse
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Alternative names
- CAG repeat protein 26 antibody
- CAGH26 antibody
- DKFZp666E117 antibody
see all
Images
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Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Datasheets and documents
References (0)
ab114857 has not yet been referenced specifically in any publications.