Anti-GW182 antibody (ab114857)

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Immunoprecipitation - Anti-GW182 antibody (ab114857)

    Key features and details

    • Rabbit polyclonal to GW182
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-GW182 antibody
      See all GW182 primary antibodies

    • Description

      Rabbit polyclonal to GW182

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IPmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

    • Immunogen

      Synthetic peptide, corresponding to a region within amino acids 1225-1275 of Human GW182 (NP_055309.2).

    • Positive control

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab114857 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 10 µg/mg of lysate.

    Target

    • Function

      Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the GW182 family.
      Contains 1 RRM (RNA recognition motif) domain.

    • Cellular localization

      Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
    • Information by UniProt

    • Database links

      • Alternative names

        • CAG repeat protein 26 antibody
        • CAGH26 antibody
        • DKFZp666E117 antibody
        • EDIE antibody
        • EMSY interactor protein antibody
        • FLJ22043 antibody
        • Glycine tryptophan protein of 182 kDa antibody
        • Glycine-tryptophan protein of 182 kDa antibody
        • GW1 antibody
        • GW182 antibody
        • GW182 autoantigen antibody
        • Hypothetical protein DKFZp566M143 antibody
        • KIAA1460 antibody
        • MGC75384 antibody
        • OTTHUMP00000122485 antibody
        • OTTHUMP00000195164 antibody
        • OTTHUMP00000195165 antibody
        • Protein GW1 antibody
        • TNR6A_HUMAN antibody
        • TNRC 6A antibody
        • TNRC6 antibody
        • Tnrc6a antibody
        • Trinucleotide repeat containing 6A antibody
        • Trinucleotide repeat containing gene 6A protein antibody
        • Trinucleotide repeat-containing gene 6A protein antibody
        see all

      Images

      • Immunoprecipitation - Anti-GW182 antibody (ab114857)
        Immunoprecipitation - Anti-GW182 antibody (ab114857)
        Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      References (0)

      Publishing research using ab114857? Please let us know so that we can cite the reference in this datasheet.

      ab114857 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      Please arrange a discount code for ab87541. Thank you.  

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      Answer

      DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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      Question

      P {margin-top:2px;margin-bottom:2px;} Dear technical team,   Our customer would like to test ICC/IF with human sample.   She searched ab70522 but, now it's not available so,   Please recommend one of your GW182 antibody to our customer.   She would like to get a discount code for it.   I look forward to hearing from you.   Best regards,

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      Answer

      Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of these products are eligible for Abreview discount programme. Let me know the product you would be interested in using I will then send you the discount code. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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