Overview

  • Product name

  • Description

    Rabbit polyclonal to GW182
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A 16 amino acid peptide from near the C terminal residues of human GW182 protein (NM_020847; Q8NDV7).

  • Positive control

    • Brain lysates.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the GW182 family.
      Contains 1 RRM (RNA recognition motif) domain.
    • Cellular localization

      Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAG repeat protein 26 antibody
      • CAGH26 antibody
      • DKFZp666E117 antibody
      • EDIE antibody
      • EMSY interactor protein antibody
      • FLJ22043 antibody
      • Glycine tryptophan protein of 182 kDa antibody
      • Glycine-tryptophan protein of 182 kDa antibody
      • GW1 antibody
      • GW182 antibody
      • GW182 autoantigen antibody
      • Hypothetical protein DKFZp566M143 antibody
      • KIAA1460 antibody
      • MGC75384 antibody
      • OTTHUMP00000122485 antibody
      • OTTHUMP00000195164 antibody
      • OTTHUMP00000195165 antibody
      • Protein GW1 antibody
      • TNR6A_HUMAN antibody
      • TNRC 6A antibody
      • TNRC6 antibody
      • Tnrc6a antibody
      • Trinucleotide repeat containing 6A antibody
      • Trinucleotide repeat containing gene 6A protein antibody
      • Trinucleotide repeat-containing gene 6A protein antibody
      see all

    Images

    • IHC image of ab84403 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84403, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:

    • Su W  et al. miR-30 disrupts senescence and promotes cancer by targeting both p16INK4A and DNA damage pathways. Oncogene N/A:N/A (2018). Read more (PubMed: 29907771) »
    • Aqil M  et al. The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference. PLoS One 8:e74472 (2013). Read more (PubMed: 24023945) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% triton x100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2012

    Question
    Answer

    DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Answer

    Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of these products are eligible for Abreview discount programme. Let me know the product you would be interested in using I will then send you the discount code. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More
    This product is known to not work in this application or species.
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Mammary cell)
    Loading amount
    50 µg
    Specification
    Mammary cell
    Treatment
    TGF beta for 3hrs
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Oct 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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