Overview

  • Product name

  • Description

    Rabbit polyclonal to GWL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GWL (internal sequence). The exact sequence is proprietary.
    Database link: Q96GX5

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; GWL transfected HEK-293T whole cell extract.
  • General notes

    Previously labelled as MASTL.

Properties

Applications

Our Abpromise guarantee covers the use of ab228753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 97 kDa.

Target

  • Function

    Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
  • Involvement in disease

    Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2700091H24Rik antibody
    • C88295 antibody
    • FLJ14813 antibody
    • GREATWALL antibody
    • Greatwall protein kinase antibody
    • GW antibody
    • GWL antibody
    • GWL_HUMAN antibody
    • hGWL antibody
    • MAST-L antibody
    • Mastl antibody
    • MGC117975 antibody
    • Microtubule associated serine/threonine kinase like antibody
    • Microtubule-associated serine/threonine-protein kinase-like antibody
    • RP11 85G18.2 antibody
    • Serine/threonine-protein kinase greatwall antibody
    • THC2 antibody
    see all

Images

  • All lanes : Anti-GWL antibody (ab228753) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : GWL transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-GWL antibody (ab228753) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

References

ab228753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228753.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up