• Product name

    Anti-GWL antibody [EPR11719(B)]
    See all GWL primary antibodies
  • Description

    Rabbit monoclonal [EPR11719(B)] to GWL
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Dog
  • Immunogen

    corresponding to Human GWL aa 750-850.

  • Positive control

    • HepG2, 293T and HeLa whole cell lysate (ab150035); HeLa cells.
  • General notes

    Previously labelled as MASTL.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab169767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 97 kDa.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
    • Involvement in disease

      Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • Post-translational

      Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2700091H24Rik antibody
      • C88295 antibody
      • FLJ14813 antibody
      • GREATWALL antibody
      • Greatwall protein kinase antibody
      • GW antibody
      • GWL antibody
      • GWL_HUMAN antibody
      • hGWL antibody
      • MAST-L antibody
      • Mastl antibody
      • MGC117975 antibody
      • Microtubule associated serine/threonine kinase like antibody
      • Microtubule-associated serine/threonine-protein kinase-like antibody
      • RP11 85G18.2 antibody
      • Serine/threonine-protein kinase greatwall antibody
      • THC2 antibody
      see all


    • All lanes : Anti-GWL antibody [EPR11719(B)] (ab169767) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 97 kDa

    • Immunofluorescence analysis of HeLa cells labeling GWL with ab169767 at a 1/100 dilution.


    ab169767 has not yet been referenced specifically in any publications.

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