Key features and details
- Non-competitive AMPA / kainate receptor antagonist
- CAS Number: 143692-48-2
- Purity: > 99%
- Soluble in water to 100 mM and in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameGYKI 53655 hydrochloride, Non-competitive AMPA / kainate receptor antagonist
DescriptionNon-competitive AMPA / kainate receptor antagonist
Non-competitive AMPA and kainate receptor antagonist. (IC50 values for inhibition of AMPA-mediated responses in cells expressing human GluR1 and GluR4 are 6 and 5 µM, respectively). Analog of GYKI 52466 (ab120336). Anticonvulsant in vivo. At higher concentrations blocks GluK3 homomeric receptors (IC50 = 63 μM) and GluK2b(R)/GluK3 heteroreceptors (IC50 = 32 μM).
Also available in simple stock solutions (ab144499) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name1-(4-Aminophenyl)-3-methylcarbamyl-4-methyl-3,4-dihydro-7,8-methylenedioxy-5H-2,3-benzodiazepine hydrochloride
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM and in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120490 has been referenced in 5 publications.
- Evans AJ et al. Assembly, Secretory Pathway Trafficking, and Surface Delivery of Kainate Receptors Is Regulated by Neuronal Activity. Cell Rep 19:2613-2626 (2017). PubMed: 28636947
- Chu HY et al. Loss of Hyperdirect Pathway Cortico-Subthalamic Inputs Following Degeneration of Midbrain Dopamine Neurons. Neuron 95:1306-1318.e5 (2017). PubMed: 28910619
- Puthussery T et al. Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina. J Neurosci 34:7611-21 (2014). PubMed: 24872565
- Chamberlain SE et al. Long-term depression of synaptic kainate receptors reduces excitability by relieving inhibition of the slow afterhyperpolarization. J Neurosci 33:9536-45 (2013). PubMed: 23719820
- Chamberlain SE et al. SUMOylation and phosphorylation of GluK2 regulate kainate receptor trafficking and synaptic plasticity. Nat Neurosci 15:845-52 (2012). PubMed: 22522402