Overview

  • Product name

    Anti-GYPB/GPB antibody [EPR15454(B)]
    See all GYPB/GPB primary antibodies
  • Description

    Rabbit monoclonal [EPR15454(B)] to GYPB/GPB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human GYPB/GPB aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P06028

  • Positive control

    • Human fetal liver, K562, mouse brain, kidney and spleen, rat brain, C6, Raw264.7, PC-12, and NIH/3T3 lysates.
  • General notes

     

     

     This product was previously labelled as GYPB

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15454(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 10 kDa).

Target

  • Function

    This protein is a minor sialoglycoprotein in erythrocyte membranes.
  • Sequence similarities

    Belongs to the glycophorin-A family.
  • Post-translational
    modifications

    The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD235b antibody
    • GLPB_HUMAN antibody
    • Glycophorin-B antibody
    • GYPB antibody
    • PAS-3 antibody
    • Sialoglycoprotein delta antibody
    • SS-active sialoglycoprotein antibody
    see all

Images

  • All lanes : Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : C6 lysate
    Lane 6 : Raw264.7 lysate
    Lane 7 : PC12 lysate
    Lane 8 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?

  • Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/10000 dilution + K562 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?

  • Anti-GYPB/GPB antibody [EPR15454(B)] (ab190351) at 1/1000 dilution + Human fetal liver at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?

References

ab190351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190351.
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