Anti-H Cadherin antibody (ab133395)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-H Cadherin antibody
See all H Cadherin primary antibodies -
Description
Rabbit polyclonal to H Cadherin -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH, from the C terminal region of Human H Cadherin.
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Positive control
- Human heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000. | |
| WB | 1/50 - 1/100. Predicted molecular weight: 78 kDa. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth. -
Tissue specificity
Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord. -
Sequence similarities
Contains 5 cadherin domains. -
Developmental stage
Expressed at higher levels in adult brain than in developing brain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1012 Human
- Omim: 601364 Human
- SwissProt: P55290 Human
- Unigene: 654386 Human
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Alternative names
- CAD13_HUMAN antibody
- Cadherin-13 antibody
- CDH13 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H Cadherin antibody (ab133395)Immunohistochemical analysis of paraffin embedded Human heart tissue labelling H Cadherin with ab133395 antibody at 10µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
References
ab133395 has not yet been referenced specifically in any publications.
