Recombinant
RabMAb

Anti-H Cadherin antibody [EPR9621] (ab167407)

Overview

  • Product name
    Anti-H Cadherin antibody [EPR9621]
    See all H Cadherin primary antibodies
  • Description
    Rabbit monoclonal [EPR9621] to H Cadherin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human H Cadherin aa 100-200. The exact sequence is proprietary.
    Database link: P55290

  • Positive control
    • Fetal heart lysate; Human heart and Human brain tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
    • Tissue specificity
      Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.
    • Sequence similarities
      Contains 5 cadherin domains.
    • Developmental stage
      Expressed at higher levels in adult brain than in developing brain.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAD13_HUMAN antibody
      • Cadherin-13 antibody
      • CDH13 antibody
      • CDHH antibody
      • H-cadherin antibody
      • Heart cadherin antibody
      • P105 antibody
      • T cad antibody
      • T Cadherin antibody
      • T-cad antibody
      • T-cadherin antibody
      • Truncated cadherin antibody
      see all

    Images

    • ab167407 showing -ve staining in Human normal liver.
    • ab167407 showing -ve staining in Human normal colon.
    • ab167407 showing -ve staining in Human normal pancreas.
    • ab167407 showing +ve staining in Human normal kidney.
    • Anti-H Cadherin antibody [EPR9621] (ab167407) at 1/1000 dilution + Fetal heart lysate at 10 µg

      Predicted band size: 78 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue, labeling H Cadherin with ab167407 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling H Cadherin with ab167407 at 1/100 dilution.

    References

    ab167407 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain, cerebellum)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.05% Tween-20
    Specification
    Brain, cerebellum
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Yi-An Yang

    Verified customer

    Submitted Jan 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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