Overview

  • Product name

    Anti-H Cadherin antibody [EPR9621]
    See all H Cadherin primary antibodies
  • Description

    Rabbit monoclonal [EPR9621] to H Cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human H Cadherin aa 100-200. The exact sequence is proprietary.
    Database link: P55290

  • Positive control

    • Fetal heart lysate; Human heart and Human brain tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
    • Tissue specificity

      Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.
    • Sequence similarities

      Contains 5 cadherin domains.
    • Developmental stage

      Expressed at higher levels in adult brain than in developing brain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAD13_HUMAN antibody
      • Cadherin-13 antibody
      • CDH13 antibody
      • CDHH antibody
      • H-cadherin antibody
      • Heart cadherin antibody
      • P105 antibody
      • T cad antibody
      • T Cadherin antibody
      • T-cad antibody
      • T-cadherin antibody
      • Truncated cadherin antibody
      see all

    Images

    • ab167407 showing -ve staining in Human normal liver.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167407 showing -ve staining in Human normal colon.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167407 showing -ve staining in Human normal pancreas.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167407 showing +ve staining in Human normal kidney.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-H Cadherin antibody [EPR9621] (ab167407) at 1/1000 dilution + Fetal heart lysate at 10 µg

      Predicted band size: 78 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue, labeling H Cadherin with ab167407 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling H Cadherin with ab167407 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Dyikanov DT  et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). Read more (PubMed: 30747044) »
    • Camolotto SA  et al. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer. Elife 7:N/A (2018). Read more (PubMed: 30475207) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain, cerebellum)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.05% Tween-20
    Specification
    Brain, cerebellum
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Yi-An Yang

    Verified customer

    Submitted Jan 05 2016

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